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Merck

UFC9010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa

別名:

10 kDa MWCO, 15 mL sample volume, Centrifugal concentrator

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この商品について

UNSPSCコード:
41104916
eCl@ss:
36100101
NACRES:
NB.24

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物質

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

品質水準

400

無菌性

non-sterile

製品種目

Amicon®

包装

pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg

メーカー／製品名

Amicon® Ultra

sustainability

Greener Alternative Product

パラメーター

15 mL sample volume

テクニック

protein extraction: suitable
protein purification: suitable

L

12.1 cm (4.807 in.)

直径

2.97 cm

ろ過面積

7.6 cm2

容量

15 mL

有効容積

≤15 mL
15 mL

ポアサイズ

10 kDa MWCO

環境により配慮した代替製品カテゴリ

Re-engineered

輸送温度

ambient

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当該品目
UFC5010UFC8010UFC201024
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC9010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC5010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC8010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

Amicon® Ultra遠心式フィルター、10 kDa MWCO sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

Millipore

UFC201024

Amicon® Ultra遠心式フィルター、10 kDa MWCO

parameter

15 mL sample volume

parameter

0.5 mL sample volume

parameter

4 mL sample volume

parameter

2 mL sample volume

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

pore size

10 kDa MWCO

pore size

10 kDa MWCO

pore size

10 kDa MWCO

pore size

10 kDa MWCO

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane

material

polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

詳細

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

アプリケーション

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

特徴および利点

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

物理的形状

Color Code: 透明　ピンク色キャップ付

法的情報

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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資料

ナノ粒子の限外ろ過

ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。

タンパク質サンプルの限外ろ過

溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

プロトコル

Extracellular Vesicle Protocol

Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.

質問

1–8/8 質問  

  1. Is there a user manual anywhere? What is the recommended g force to be used with this filter?

    1 回答

    1. The spin conditions for a swinging-bucker rotor are 4000 x g and for a fixed-angle rotor are 5000 x g, at a time range of 10 - 60 minutes, based on molecular weight cut-off.

      Please see the link below to review the Product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/181/pr05035wrev1023-ms.pdf

      役に立ちましたか？

  2. Is there a recommended temperature for the amicon ultra-15? I would like to concentrate protein at 0 degrees C. Also, is glycerol able to pass through the 30kD filter? The enzyme I want to concentrate is in 50% glycerol. Thanks!

    1 回答

    1. No data is available on the maximum or minimum temperature for using the Amicon Ultra-15. Results shown in the user guides are based on experiments conducted at approximately 25°C, not with refrigerated centrifuges. Some users work at 4°C, but filtration times are longer. No studies have been performed at 0°C. Glycerol is compatible with this product.

      役に立ちましたか？

  3. Can I concentrate cell-propagated respiratory syncytial virus stock using the Amicon® Ultra Centrifugal Filter, 10 kDa MWCO? what is your though and suggestion?

    1 回答

    1. Concentrating cell-propagated respiratory syncytial virus stock with this product has not been studied. However, it can be attempted, as other viruses such as SARS, AAV, and Lenti have been successfully concentrated. The product concentrates substances larger than the molecular weight cut-off of the membrane. It is recommended to consult online research articles using keywords like "Amicon and respiratory syncytial virus" on platforms such as PubMed or Google Scholar.

      役に立ちましたか？

  4. Do you make a miniature version of the Amicon® Ultra Centrifugal Filter for 1.5ml samples?

    1 回答

    1. Yes, product UFC5010, the Amicon Ultra 2.0 for samples of 0.5 - 2.0 mL, will accommodate a 1.5 mL sample size. Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/mm/ufc5010

      役に立ちましたか？

  5. Does ultrafiltration of protein samples result in lower migration on an SDS-PAGE gel?

    1 回答

    1. ​Yes, ultrafiltration of protein samples can potentially lead to lower migration on an SDS-PAGE gel.During the ultrafiltration process, the desalting or buffer exchange step may remove or reduce sodium chloride (NaCl), leading to lower protein migration on the gel. To prepare protein samples for gel electrophoresis, using an Amicon Ultra (AU) device or a suitable desalting/buffer exchange method is recommended.

      役に立ちましたか？

  6. Hello, How much filters represent 1pkg? Only one filter? A full pack? Thank you,

    1 回答

    1. Amicon® Ultra-15 Centrifugal Filter Unit comes in 8, 24, and 96/pk. the pack size is indicated in the last two digits of the catalog numbers.

      役に立ちましたか？

  7. Hello, I'm using UFC903096 (Amicon-15, 30K) and am trying to exchange and concentrate without over-concentrating <500uL ... Would you have a table of the volume to add to the filtrate collector to ensure ~ final product volumes. Thanks!

    1 回答

    1. This product is suitable for sample volumes of less than 15 mL. Please see the link below to review the estimated volumes:
      https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#howtoselect

      役に立ちましたか？

  8. How to wash the column after one time used for the buffer exchange or for protein concentration. Is any protocol please share??

    1 回答

    1. We do not recommend reusing the device because eventually the membrane will clog completely. We have yet to record an efficient way to clean the pores of the membrane without damaging the membrane itself.

      役に立ちましたか？

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