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Merck

X100

Triton X-100

laboratory grade

Triton™ X-100

別名:

4-(1,1,3,3-テトラメチルブチル)フェニル-ポリエチレングリコール, t-オクチルフェノキシポリエトキシエタノール, ポリエチレングリコール tert-オクチルフェニルエーテル

ログインで組織・契約価格をご覧ください。

サイズを選択してください

5 ML

¥4,130

100 ML

¥11,900

500 ML

¥15,100

1 L

¥21,600

1 GAL

¥56,900

5 GAL

¥184,000

¥4,130


在庫あり出荷可能詳細


バルクの問い合わせ

この商品について

化学式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS番号:
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
2315025

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製品名

Triton X-100, laboratory grade

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

biological source

synthetic

grade

laboratory grade

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

mp

6  °C (43 °F)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

Quality Level

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当該品目
X114T92841.08643
Triton™ X-100 EMPROVE® EXPERT Ph Eur

SAFC

1.08643

Triton X-100

grade

laboratory grade

grade

laboratory grade

grade

-

grade

-

form

liquid

form

-

form

liquid

form

liquid

aggregation number

100-155

aggregation number

-

aggregation number

100-155

aggregation number

100-155

biological source

synthetic

biological source

-

biological source

synthetic

biological source

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

500

CMC

0.2-0.9 mM (20-25°C)

CMC

0.2 mM (20-25°C)

CMC

0.2-0.9 mM (20-25°C)

CMC

0.2-0.9 mM (20-25°C)

Application

Triton-X は以下に使用されています:
  • 免疫蛍光染色のための細胞の透過処理[1]
  • ウェスタンブロット解析における可溶化バッファーの成分[1]
  • 電子顕微鏡用イムノゴールド標識における細胞切片の調製のための、トリス緩衝食塩水の成分[1]

Biochem/physiol Actions

穏やかな非変性条件下での膜成分の回収に広く用いられている非イオン性界面活性剤です。

Features and Benefits

  • 細胞生物学および生化学の研究に最適
  • 非イオン性非変性洗浄剤
  • 優れた湿潤剤かつ乳化剤
  • 汎用性の高い界面活性剤

General description

Triton X-100 は溶血作用を持っており[2]、DNA 抽出に使用されます。[3]
Triton X-100 は、一般的な非イオン性の界面活性剤であり、タンパク質の可溶化を必要とする生化学アプリケーションにおいてしばしば使用される乳化剤です。非変性の、比較的マイルドな界面活性剤であると考えられており、日常的に添加される試薬として、多くの文献に報告されています。タンパク質や細胞内小器官を抽出するための細胞の可溶化に使用されます。また、トランスフェクションのための、生細胞の膜透過処理にも使用されます。[4][5]

Other Notes

メルクの生化学製品に関する追加情報については、このフォームに必要事項を入力してください。

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

José Francisco Lima-Barbero et al.
Experimental & applied acarology, 78(4), 555-564 (2019-08-02)
Blood-feeding ectoparasites constitute a growing burden for human and animal health, and animal production worldwide. In particular, mites (Acari: Gamasida) of the genera Dermanyssus (Dermanyssidae) and Ornithonyssus (Macronyssidae) infest birds and cause gamasoidosis in humans. The tropical fowl mite, Ornithonyssus
Knockdown of miR-210 decreases hypoxic glioma stem cells stemness and radioresistance
Yang W, et al.
Experimental Cell Research, 326(1), 22-35 (2003)
alpha -Synuclein Is Degraded by Both Autophagy and the Proteasome
Webb LJ, et al.
The Journal of Biological Chemistry, 278(27), 25009-25013 (2003)
Masoumeh Zangeneh et al.
Materials science & engineering. C, Materials for biological applications, 103, 109739-109739 (2019-07-28)
The purpose of this study was to investigate the radiation dose enhancement effects of gadolinium-doped zinc oxide nanoparticles (Gd-doped ZnO NPs) under the megavoltage (MV) X-ray irradiation. ZnO NPs have preferred photocatalytic properties under UV light for cancer killing. UV
Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)

資料

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

プロトコル

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

This page shows how to solubilize membrane proteins with products from Cytiva.

質問

1–10/20 質問  
  1. Salve Questo è uno standard Certificato oppure un materiale di riferimento? Posso usarlo come standard per retta di taratura in HPLC_MS?

    1 回答
    1. This product is not a certified reference material, nor has it been tested for use in HPLC/MS. As Triton X-100 is typically avoided in Mass Spectrometry, there is no analytical standard currently that is qualified for this application. The end user would have to determine suitability for use in HPLC/MS. However, this material is referenced in an LC/MS publication by Shimadzu. Please see the link below to review:
      https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/white_papers/13844/apo21759.pdf

      役に立ちましたか?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      役に立ちましたか?

  3. What kind of change will be observed if heat up to 55C? Will it attack stacked Al and Ti metal layer? Can it mix with diluted nitric acid to etch Cu/NiV?

    1 回答
    1. Heating this product to 55°C may lead to changes in viscosity, solubility, or performance characteristics. There is no indication that this product can attack aluminum and titanium metal layers. This product is generally compatible with diluted nitric acid. While no specific incompatibility is reported, the combination may alter the properties of both the surfactant and the acid, potentially affecting the performance of either in applications such as cleaning or etching. Additionally, it is important to consider the concentration and the potential for foaming or other reactions during mixing.

      役に立ちましたか?

  4. Is this product nuclease-free?

    1 回答
    1. 役に立ちましたか?

  5. How long is Triton X-100 stable at -80 degrees in buffer?

    1 回答
    1. At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.

      役に立ちましたか?

  6. If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?

    1 回答
    1. Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
      concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
      https://www.sigmaaldrich.com/product/sigma/t8787
      https://www.sigmaaldrich.com/product/mm/648466
      https://www.sigmaaldrich.com/product/sigma/93443

      役に立ちましたか?

  7. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか?

  8. What are the storage conditions for the Triton 100x? It's not in the COA

    1 回答
    1. This product may be be stored in room temperature.

      役に立ちましたか?

  9. What is the correct CAS number for Triton X-100, 9002-93-1 or 9036-19-5?

    1 回答
    1. CAS 9036-19-5 is a generic CAS number and is correct for Triton® X-100. With this number, neither the substitution pattern of the aromatic ring (para, meta, ortho), nor the amount of the repeating units of the oxyethylene units is specified or described. Triton® X-100 with CAS 9002-93-1 is also correct but it defines only the para-substitution.

      役に立ちましたか?

  10. Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?

    1 回答
    1. Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.

      役に立ちましたか?

1–10/20 質問  

レビュー

アクティブなフィルタ

  1. Lillehammer, Norway
    • 2件のレビュー
    • 4件の投票
    星5/5個です。

    M.Sc.

    We use the Product for surface cleaning of artworks. Works as planned.

    Googleで翻訳する

    役に立ちましたか?

  2. 1件の評価のみのレビュー

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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