A0561
ACCUSPIN™ System-Histopaque®-1077
sterile-filtered, density: 1.077 g/mL
スキップする
製品名
ACCUSPIN™ System-Histopaque®-1077, sterile-filtered, density: 1.077 g/mL
sterility
aseptically filled
form
solution
shelf life
Expiry date on the label.
packaging
package of 100 tubes
technique(s)
cell culture | mammalian: suitable
density
1.077 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
Quality Level
類似した製品をお探しですか？ 訪問 製品比較ガイド
関連するカテゴリー
1 of 4
当該品目 | A7054 | A1805 | A6929 |
|---|---|---|---|
| form solution | form solution | form tubes | form solution |
| density 1.077 g/mL | density 1.077 g/mL | density - | density 1.077 g/mL |
| sterility aseptically filled | sterility aseptically filled | sterility sterile; irradiated | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| Quality Level 400 | Quality Level 400 | Quality Level 200 | Quality Level 400 |
| shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life - | shelf life Expiry date on the label. |
Application
ACCUSPIN System-HISTOPAQUE-1077には、特別にデザインされた遠心チューブを使用しており、このチューブには多孔質高密度ポリエチレンバリア(「フリット」)によって区切られた2つのチャンバーがあります。下のチャンバーにはHISTOPAQUE-1077が入っており、分離媒体と混ざるおそれなしに、抗凝固剤を添加した血液を入れることができます。遠心分離すると、全血はフリットを通ってその下のHISTOPAQUE-1077と接触し、血液成分が明確に分離されます。赤血球が凝集し、顆粒球が若干高張になることで沈降速度が上昇し、その結果、ACCUSPINチューブの底に沈殿します。リンパ球をはじめとする単核細胞、すなわち単球は、血漿とHISTOPAQUE-1077の界面に保持されます。
ACCUSPIN System-Histopaque-1077 is used for the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradient separation media. Histopaque-1077 has been used for:
- human lymphocyte antigen (HLA) typing
- initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes.
- preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
- isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)citation
- isolation of human lymphocytescitation
- isolation of breast cancer circulating tumor cells (CTCs) from the whole bloodcitation
Biochem/physiol Actions
The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
Features and Benefits
ACCUSPIN™ System-Histopaque®-1077 offers numerous advantages, such as:
- Optimal recovery of viable cells
- Permits selective separation of blood cell types
- Prevents cell distortion
- Preserves cell viability
- Minimal extraneous cell interference
- Reproducible performance from batch to batch
- Histopaque is sterile-filtered and endotoxin-tested
- Stable at 2-8°C for at least 2 years when protected from light
General description
ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 50 mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). Each tube is aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
保管分類
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Janet C Lindow et al.
PLoS neglected tropical diseases, 6(7), e1742-e1742 (2012-07-21)
The four dengue virus serotypes (DENV-1-DENV-4) have a large impact on global health, causing 50-100 million cases of dengue fever annually. Herein, we describe the first kinetic T cell response to a low-dose DENV-1 vaccination study (10 PFU) in humans.
Tiffany A Greenwood et al.
PloS one, 7(1), e29630-e29630 (2012-01-19)
While it is clear that schizophrenia is highly heritable, the genetic basis of this heritability is complex. Human genetic, brain imaging, and model organism studies have met with only modest gains. A complementary research tactic is to evaluate the genetic
Nicolas Antoine-Moussiaux et al.
PloS one, 4(5), e5728-e5728 (2009-05-30)
In a recent study dealing with a mouse model of Trypanosoma evansi-associated disease, a remarkable synchrony between the parasitaemia peak and the white-blood-cell count nadir was noticed. The present study was designed to establish whether there is a direct causal
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）