Ensuring that weighing instruments are calibrated is essential for accurate measurements. This approach should be acceptable for preparing the desired concentrations.
D9542
DAPI
for nucleic acid staining
DAPI
別名:
4′,6-ジアミジノ-2-フェニルインドール 二塩酸塩, 2-(4-アミジノフェニル)-6-インドールカルバミジン 二塩酸塩, DAPI 二塩酸塩
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1 mg | 在庫fromsìså¬368 | ￥3,200 |
5 mg | 在庫fromsìså¬368 | ￥15,700 |
10 mg | 在庫fromsìså¬368 | ￥23,800 |
50 mg | カートの在庫状況を確認する | ￥77,600 |
この商品について
実験式（ヒル表記法）:
C16H15N5 · 2HCl
CAS番号:
分子量:
350.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
grade
Molecular Biology
Quality Segment
assay
≥98% (HPLC)
form
powder
technique(s)
transfection: suitable
solubility
H2O: 20 mg/mL, PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O at 1 mM
fluorescence
λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
General description
DAPI（4',6-ジアミジノ-2-フェニルインドールジヒドロクロリド）は、DNAと結合する細胞透過性の蛍光色素です。
熱または超音波処理が必要になる場合があります。室温または 4 ℃ で暗所に保存した溶液は、2 ～ 3 週間安定です。
Application
DAPIは、アガロースゲル中のDNA染色において、エチジウムブロマイドよりも数倍高感度です。
DAPIはアガロ-スゲル中の臭化エチジウムによるDNA染色よりも数倍高い感度を示します。DNAの蛍光フットプリンティングにも使用可能で、また、二本鎖形成を特異的に可視化するためブロッティングの際にアニ-リングしているプロ-ブを検出でき、フロ-サイトメトリ-を使用することでアポト-シス中のDNA変化の調査やDNA量の分析が可能です。DAPI染色は、マイコプラズマに対する高感度かつ特異的な検出方法であることも示されています。
Biochem/physiol Actions
細胞透過性を有し、副溝に結合するDNA用蛍光プロ-ブです。二本鎖DNAの副溝に(ATリッチなDNAを優先して)結合し、DAPI単独よりも約20倍強い蛍光を発する安定な複合体を形成します。
Disclaimer
遮光保存してください。
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当該品目 | |||
|---|---|---|---|
| description for nucleic acid staining | description 4′,6-Diamidine-2′-phenylindole dihydrochloride | description For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL | description Cell-permeable DNA-binding dye. |
| technique(s) transfection: suitable | technique(s) - | technique(s) transfection: suitable | technique(s) - |
| grade Molecular Biology | grade - | grade - | grade - |
| suitability suitable for fluorescence | suitability - | suitability suitable for nucleic acid staining | suitability - |
| fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex ~359 nm, λem ~461 nm |
| form powder | form powder | form liquid | form solid |
| solubility H2O: 20 mg/mL, PBS: insoluble | solubility - | solubility water: soluble | solubility water: 5 mg/mL, ethanol: soluble, methanol: soluble |
Still not finding the right product?
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signalword
Warning
hcodes
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
target_organs
Respiratory system
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can I dissolve 1mg in 50ul of H2O to adjust the concentration to 20mg/ml, then dilute it to 0.5ug/ml~5ug/ml and apply it to the cells?
1 回答-
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Can this product be used to stain bacteria (E.coli) chromosome?
1 回答-
DAPI is suitable for staining bacterial chromosomes, including those of E. coli.
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What is the working dilution of DAPI that should be used to stain the nuclei of human cells?
1 回答-
Typical working concentration of DAPI used in most applications is 0.1μg/mL-10μg/mL. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/774/984/d9564pis.pdfPlease see the link below to review a sample protocol from Cold Spring Harbor:
https://cshprotocols.cshlp.org/content/2011/1/pdb.prot5556.long役に立ちましたか？
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what kind of the water used to dissolve DAPI? and what's the temperature should be dissolve the water?
1 回答-
This product is soluble at 20 mg/mL in water at ambient temperature. No specific water type or treatment is required. Deionized water is typically used for laboratory applications.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdf役に立ちましたか？
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What is the suggested concentration for product D9542-1MG?
1 回答-
The suggested concentration for product D9542-1MG, DAPI, is in the range of 0.5 to 5 ug/ml.
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Can the stain concentration be used for staining fish scales and any animal cells for ICC?
1 回答-
Yes, DAPI can be used to stain fish scale nuclei. While specific data regarding the concentration and duration for staining fish scale nuclei is not available, it is suggested to start with a working concentration of around 0.5 mg/ml and optimize the staining for each specific cell type, as different cells may uptake the dye at varying rates. It is also advisable to try staining at both higher and lower concentrations. For staining mammalian cells, lower concentrations are typically used.
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What is the suitable DAPI for immunofluorescence staining of free-floating rat brain sections?
1 回答-
Several DAPI products are suitable for staining free-floating rat brain sections. The same DAPI used for staining mycoplasma, cultured cells, or other cell types can be used for this purpose. Any of the DAPI products listed on the website should be suitable for this use.
役に立ちましたか？
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肝細胞を染める染色プロトコルを教えてください。
1 回答-
Please see the link below to review the product datasheet, which includes basic protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfA protocol specifically for hepatocyte staining is not available. However, the publication shown at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671579/役に立ちましたか？
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Aside from the applications described on the product page, how is D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), used?
1 回答-
DAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high. It appears that DAPI uses an intercalating binding mode with RNA that is AU selective (Biochem., 31, 3103 (1992)). Selectivity of DAPI for DNA over RNA is greater than that shown by ethidium bromide and propidium iodide (Nucleic Acid Research, 6, 3535 (1979))
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What is the stability of D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride?
1 回答-
Product D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride has a stability of 3 years as supplied. The product in solution is also stable in the dark at 4°C and showed no signs of degradation after three weeks at room temperature in the dark. Material exposed to light showed significant degradation. We have developed an HPLC method for our DAPI products that shows reassay results from July 2012 for lots that were originally released for sale in December 2008, for both D9542 (DAPI dihydrochloride) and D9564 (DAPI dilactate).
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