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Merck

D9542

DAPI

for nucleic acid staining

DAPI

別名:

4′,6-ジアミジノ-2-フェニルインドール 二塩酸塩, 2-(4-アミジノフェニル)-6-インドールカルバミジン 二塩酸塩, DAPI 二塩酸塩

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1 MG

￥1,600

5 MG

￥11,775

10 MG

￥17,850

50 MG

￥54,320

￥1,600

単価￥3,200割引50%

在庫あり出荷可能詳細

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この商品について

実験式（ヒル表記法）:
C16H15N5 · 2HCl
CAS番号:
28718-90-3
分子量:
350.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24894305
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
MFCD00012681

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製品名

DAPI, for nucleic acid staining

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

grade

Molecular Biology

assay

≥98% (HPLC)

form

powder

technique(s)

transfection: suitable

solubility

H2O: 20 mg/mL
PBS: insoluble

ε (extinction coefficient)

30 at 263 nm in H2O at 1 mM

fluorescence

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

300

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当該品目
10236276001268298MBD0020
DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

DAPI 4′,6-Diamidine-2′-phenylindole dihydrochloride

Roche

10236276001

DAPI

DAPI, Dihydrochloride Cell-permeable DNA-binding dye.

Sigma-Aldrich

268298

DAPI, Dihydrochloride

4′,6-ジアミジノ-2-フェニルインドール 二塩酸塩 For Nuclear counterstain in Fluorescence Microscopy

Sigma-Aldrich

MBD0020

4′,6-ジアミジノ-2-フェニルインドール 二塩酸塩

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

suitable for nucleic acid staining

grade

for molecular biology

grade

-

grade

-

grade

-

technique(s)

transfection: suitable

technique(s)

-

technique(s)

-

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex ~359 nm, λem ~461 nm

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

form

powder

form

powder

form

solid

form

liquid

solubility

H2O: 20 mg/mL, PBS: insoluble

solubility

-

solubility

water: 5 mg/mL, ethanol: soluble, methanol: soluble

solubility

-

Application

DAPIは、アガロースゲル中のDNA染色において、エチジウムブロマイドよりも数倍高感度です。
DAPIはアガロ-スゲル中の臭化エチジウムによるDNA染色よりも数倍高い感度を示します。DNAの蛍光フットプリンティングにも使用可能で、また、二本鎖形成を特異的に可視化するためブロッティングの際にアニ-リングしているプロ-ブを検出でき、フロ-サイトメトリ-を使用することでアポト-シス中のDNA変化の調査やDNA量の分析が可能です。DAPI染色は、マイコプラズマに対する高感度かつ特異的な検出方法であることも示されています。
次のことに適しています
  • アガロースゲル中のDNA染色
  • アポトーシスにおけるDNA変化の解析
  • マイコプラズマの検出
  • DNAのフォトフットプリンティング
  • 細胞の免疫蛍光染色

DAPIは以下のことに使用されています：-
  • 微生物コンタミネーションの迅速モニタリング[1]
  • クロモソームバンディング技術[2]
  • アポトーシス細胞の検出[3]
  • Bacillus subtilis DNAにおけるDisA（DNAインテグリティスキャニングタンパク質）の移動を追跡するための蛍光顕微鏡解析[4]
  • 成熟花粉粒の染色（0.5 mg/mL）[5]

Biochem/physiol Actions

細胞透過性を有し、副溝に結合するDNA用蛍光プロ-ブです。二本鎖DNAの副溝に(ATリッチなDNAを優先して)結合し、DAPI単独よりも約20倍強い蛍光を発する安定な複合体を形成します。

Disclaimer

遮光保存してください。

General description

DAPI（4',6-ジアミジノ-2-フェニルインドールジヒドロクロリド）は、DNAと結合する細胞透過性の蛍光色素です。
熱または超音波処理が必要になる場合があります。室温または 4 ℃ で暗所に保存した溶液は、2 ～ 3 週間安定です。

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Related product

製品番号
詳細
価格

08168

Timestrip Plus™ 8°C

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

保管分類

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
0000502555
0000502555
0000496874
0000496874
0000486019

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Yan Li et al.
Toxicology letters, 154(3), 225-233 (2004-10-27)
Excessive exposure to synthetic and endogenous estrogens has been associated with the development of cancer in several tissues including the breast. 4-Hydroxyequilenin (4-OHEN), a major catechol metabolite of equine estrogens present in Premarin, an estrogen replacement formulation, has been shown
Michal Bejerano-Sagie et al.
Cell, 125(4), 679-690 (2006-05-23)
In response to DNA damage, cells activate checkpoint signaling cascades to control cell-cycle progression and elicit DNA repair in order to maintain genomic integrity. The sensing and repair of lesions is critical for Bacillus subtilis cells entering the developmental process
J Kapuscinski
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 70(5), 220-233 (1995-09-01)
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and
S Ituarte et al.
Genetica, 122(2), 199-206 (2004-12-22)
Male karyotype and meiosis of Tenagobia fuscata (Corixoidea, Micronectidae) are studied. The species possesses a male diploid chromosome number 2n = 28 + XY, holokinetic chromosomes, absence of m chromosomes and an achiasmatic male meiosis. Autosomes divide pre-reductionally while the
Christy A Itoga et al.
The Journal of comparative neurology, 527(15), 2474-2487 (2019-03-13)
Corticotropin-releasing hormone (CRH) is an essential, evolutionarily-conserved stress neuropeptide. In addition to hypothalamus, CRH is expressed in brain regions including amygdala and hippocampus where it plays crucial roles in modulating the function of circuits underlying emotion and cognition. CRH+ fibers
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Product Information Sheet - D9542

Instructions

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質問

1–10/12 質問  

  1. Can this product be used to stain bacteria (E.coli) chromosome?

    1 回答

    1. DAPI is suitable for staining bacterial chromosomes, including those of E. coli.

      役に立ちましたか？

  2. What is the working dilution of DAPI that should be used to stain the nuclei of human cells?

    1 回答

    1. Typical working concentration of DAPI used in most applications is 0.1μg/mL-10μg/mL. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/774/984/d9564pis.pdf

      Please see the link below to review a sample protocol from Cold Spring Harbor:
      https://cshprotocols.cshlp.org/content/2011/1/pdb.prot5556.long

      役に立ちましたか？

  3. what kind of the water used to dissolve DAPI? and what's the temperature should be dissolve the water?

    1 回答

    1. This product is soluble at 20 mg/mL in water at ambient temperature. No specific water type or treatment is required. Deionized water is typically used for laboratory applications.

      Please see the link below to review the product data sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdf

      役に立ちましたか？

  4. Can the stain concentration be used for staining fish scales and any animal cells for ICC?

    1 回答

    1. Yes, DAPI can be used to stain fish scale nuclei. While specific data regarding the concentration and duration for staining fish scale nuclei is not available, it is suggested to start with a working concentration of around 0.5 mg/ml and optimize the staining for each specific cell type, as different cells may uptake the dye at varying rates. It is also advisable to try staining at both higher and lower concentrations. For staining mammalian cells, lower concentrations are typically used.

      役に立ちましたか？

  5. What is the suggested concentration for product D9542-1MG?

    1 回答

    1. The suggested concentration for product D9542-1MG, DAPI, is in the range of 0.5 to 5 ug/ml.

      役に立ちましたか？

  6. What is the suitable DAPI for immunofluorescence staining of free-floating rat brain sections?

    1 回答

    1. Several DAPI products are suitable for staining free-floating rat brain sections. The same DAPI used for staining mycoplasma, cultured cells, or other cell types can be used for this purpose. Any of the DAPI products listed on the website should be suitable for this use.

      役に立ちましたか？

  7. 肝細胞を染める染色プロトコルを教えてください。

    1 回答

    1. Please see the link below to review the product datasheet, which includes basic protocols:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdf

      A protocol specifically for hepatocyte staining is not available. However, the publication shown at the link below may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671579/

      役に立ちましたか？

  8. Aside from the applications described on the product page, how is D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), used?

    1 回答

    1. DAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high.  It appears that DAPI uses an intercalating binding mode with RNA that is AU selective (Biochem., 31, 3103 (1992)). Selectivity of DAPI for DNA over RNA is greater than that shown by ethidium bromide and propidium iodide (Nucleic Acid Research, 6, 3535 (1979))

      役に立ちましたか？

  9. How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be dissolved?

    1 回答

    1. This product is soluble in water at 20 mg/mL, but may require heat.  DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).

      役に立ちましたか？

  10. How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be stored once in solution, and for how long would it be good?

    1 回答

    1. Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation.  At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks.  For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.

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