- A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research.
A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research.
Metabolomics : Official journal of the Metabolomic Society (2014-07-25)
Christoph Bueschl, Bernhard Kluger, Marc Lemmens, Gerhard Adam, Gerlinde Wiesenberger, Valentina Maschietto, Adriano Marocco, Joseph Strauss, Stephan Bödi, Gerhard G Thallinger, Rudolf Krska, Rainer Schuhmacher
PMID25057268
ABSTRAKT
Many untargeted LC-ESI-HRMS based metabolomics studies are still hampered by the large proportion of non-biological sample derived signals included in the generated raw data. Here, a novel, powerful stable isotope labelling (SIL)-based metabolomics workflow is presented, which facilitates global metabolome extraction, improved metabolite annotation and metabolome wide internal standardisation (IS). The general concept is exemplified with two different cultivation variants, (1) co-cultivation of the plant pathogenic fungi
MATERIAŁY
Numer produktu
Marka
Opis produktu
Sigma-Aldrich
Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
Sigma-Aldrich
Citric acid, anhydrous, suitable for cell culture, suitable for plant cell culture
Supelco
Monobasic Potassium Phosphate, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Citric acid, meets analytical specification of Ph. Eur., BP, USP, E330, anhydrous, 99.5-100.5% (based on anhydrous substance)
Supelco
Citric acid, Anhydrous, Pharmaceutical Secondary Standard; Certified Reference Material
Reserpine, European Pharmacopoeia (EP) Reference Standard
Citric acid, anhydrous, European Pharmacopoeia (EP) Reference Standard