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Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Artificial cells, nanomedicine, and biotechnology (2013-06-26)
Ceren Erdem, Derya Koyuncu Zeybek, Gözde Aydoğdu, Bülent Zeybek, Sule Pekyardımcı, Esma Kılıç
ABSTRAKT

In the present work, we designed an amperometric glucose biosensor based on nickel oxide nanoparticles (NiONPs)-modified carbon paste electrode. The biosensor was prepared by incorporation of glucose oxidase and NiONPs into a carbon paste matrix. It showed good analytical performances such as high sensitivity (367 μA mmolL(-1)) and a wide linear response from 1.9×10(-3) mmolL(-1) to 15.0 mmolL(-1) with a limit of detection (0.11 μmolL(-1)). The biosensor was used for the determination of glucose in human serum samples. The results illustrate that NiONPs have enormous potential in the construction of biosensor for determination of glucose.

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Sigma-Aldrich
L-Ascorbic acid, FCC, FG
Sigma-Aldrich
Nickel(II) oxide, green, −325 mesh, 99%
Sigma-Aldrich
D-Glucose-12C6, 16O6, 99.9 atom % 16O, 99.9 atom % 12C
Supelco
D-(+)-Glucose, analytical standard
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L-Ascorbic acid, analytical standard
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L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)
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L-Ascorbic acid, BioUltra, ≥99.5% (RT)
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Sodium phosphate monobasic dihydrate, BioUltra, Molecular Biology, ≥99.0% (T)
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D-(+)-Glucose, tested according to Ph. Eur.
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D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
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L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)
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Sodium phosphate monobasic dihydrate, purum p.a., crystallized, ≥99.0% (T)
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L-Ascorbic acid, 99%
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Urea-12C, 99.9 atom % 12C
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Nickel(II) oxide, 99.99% trace metals basis
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L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
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L-Ascorbic acid, meets USP testing specifications
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L-Ascorbic acid, BioXtra, ≥99.0%, crystalline
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Urea solution, 40 % (w/v) in H2O
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D-(+)-Glucose, Hybri-Max, powder, BioReagent, suitable for hybridoma
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D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
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D-(+)-Glucose, ACS reagent
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D-(+)-Glucose, ≥99.5% (GC), BioXtra
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D-(+)-Glucose, ≥99.5% (GC)
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Urea solution, BioUltra, ~8 M in H2O
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Nickel(II) oxide, nanowires, diam. × L ~20 nm × 10 μm
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