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1.05628

Supelco

HPTLC Silica gel 60 F₂₅₄

pkg of 25 plates, plate L × W 10 cm × 10 cm, glass support

storage conditions

no restrictions.

material

glass support
silica gel 60 matrix

Quality Level

packaging

pkg of 25 plates

application(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

10 cm × 10 cm

storage temp.

2-30°C

General description

25 Glass plates 10 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Application

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.

Linkage

Replaces: 5628-5; 5628

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

Certificate of Analysis

Certificate of Origin

Protocols
The modern thin-layer chromatography (TLC) offers advantages also in the field of cosmetics to quickly and simultaneously analyze samples with densitometric detection.
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