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HPTLC plates, Silica gel 60 F₂₅₄

pkg of 25 plates, plate L × W 10 cm × 10 cm, glass support


glass support
silica gel 60 matrix

Quality Level


binder Organic Polymer
fluorescent indicator


pkg of 25 plates


thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

10 cm × 10 cm

particle size

5-6 μm

pore size

60 Å medium pore diameter

storage temp.


General description

25 Glass plates 10 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.


Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.


Replaces: 5628-5; 5628

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

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