Freeze-thaw cycles may lead to degradation of added phosphatase and protease inhibitors. It is highly recommended to prepare the cocktail just prior to application.
20-188
RIPA Lysis Buffer, 10X
100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.
Synonym(s):
lysis buffer
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100 ML
$137.00
$137.00
Estimated to ship onJuly 21, 2025
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100 ML
$137.00
About This Item
UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22
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$137.00
Estimated to ship onJuly 21, 2025
form
liquid
packaging
pkg of 100 mL
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
ambient
1 of 4
This Item | R0278 | 11814389001 | B6429 |
|---|---|---|---|
| technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunoprecipitation (IP): suitable | technique(s) - | technique(s) Southern blotting: suitable |
| form liquid | form liquid | form solution | form solution |
| packaging pkg of 100 mL | packaging pkg of 50 mL, pkg of 500 mL | packaging pkg of 100 mL | packaging - |
| manufacturer/tradename Upstate® | manufacturer/tradename - | manufacturer/tradename Roche | manufacturer/tradename - |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
General description
Radioimmunoprecipitation assay (RIPA) Buffer aids in cell lysis and solubilization of proteins from both adherent and suspension-cultured mammalian cells. In addition, this buffer helps in relevant protein-protein interactions studies by reducing non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur.
Application
Physical form
0.5M Tris-HCl, pH 7.4, 1.5M NaCl, 2.5% deoxycholic acid, 10% NP-40, 10mM EDTA.
Storage and Stability
6 months at room temperature
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Can I prepare 10 ml of 1x RIPA buffer and add phosphatase and protease inhibitor then aliquot it to 1 ml Eppendorf tubes and store at -20 for use?
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Can this lysis extract protein from the Nucleus?
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RIPA Buffer enables the extraction of cytoplasmic, membrane, and nuclear proteins. Certain proteins are more soluble in RIPA buffer than others.
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How long can 1x RIPA lysis buffer be stored with or without the protease?
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This product is stable at room temperature for 6 months from date of shipment. Protease and phosphatase inhibitors should be added immediately prior to use.
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I used the 10X RIPA buffer to lyse my cultured cells. How can I do next? Will it affect the protein quantification by using BCA assay kit? Also, will it affect the SDS-PAGE and Western blot?
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Protein samples prepared from cell culture lysates using this 1X RIPA buffer are typically compatible with downstream applications such as BCA assay, SDS-PAGE, and Western blotting. The concentrations of salts and detergents present in the 1X RIPA buffer are relatively low, minimizing interference with these techniques.
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Can we store it a 4 degree, if we want to use it after 6 months?
1 answer-
Studies have not been performed to determine if refrigerated storage will extend the shelf life of this product. The end user would have to determine suitability if used after the recommended 6 months. Note that product R0278, a 1X Ripa Buffer, is stored refrigerated and includes a warranty of 1 year from the date of shipment. Please see the link below to review this product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdfHelpful?
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Someone in lab left the 10X RIPA Buffer in room temperature for overnight. Will it affect the quality of the buffer? Could we continue to use it?
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This product does not require refrigeration and is stable at room temperature for up to 6 months.
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Do I used distilled water to dilute to 1X?
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The 10X RIPA Lysis Buffer should be diluted with nine parts sterile, distilled water.
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Do I have to dilute to 1X for protein isolation?
1 answer-
Yes, RIPA lysis buffer should be diluted to 1X prior to use.
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