This product has not been tested for use in cell culture. Note that product C1740, while not tested for cell culture applications, has been used successfully by researchers. Please see the link below to review this product, including the peer reviewed papers:
https://www.sigmaaldrich.com/product/sigma/c1740
204876
Complement C1q, Human
Complement C1q, Human, CAS 80295-33-6, is a native C1q complement component composed of 18 polypeptide chains consisting of three nonidentical A, B, C subunits of 29, 26, and 19 kDa, respectively.
Synonym(s):
Complement C1q, Human
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About This Item
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Quality Level
assay
≥90% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
impurities
IgG, IgA, IgM, C3, C4, albumin, fibrinogen, α₂-macroglobulin, trace
shipped in
wet ice
storage temp.
−70°C
1 of 4
This Item | 204888 | C1740 | 204896 |
|---|---|---|---|
| assay ≥90% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥85% (SDS-PAGE) |
| form liquid | form liquid | form liquid | form liquid |
| impurities IgG, IgA, IgM, C3, C4, albumin, fibrinogen, α₂-macroglobulin, trace | impurities IgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9, trace | impurities - | impurities IgG, IgA, IgM, albumin, C3, C4, C7, C9, trace |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition - | storage condition OK to freeze, avoid repeated freeze/thaw cycles |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Native, human C1q complement component. Glycoprotein composed of 18 polypeptide chains consisting of three nonidentical subunits, A, B, and C, of M.W. 29 kDa, 26 kDa, and 19 kDa, respectively. Present in normal human serum at 70 µg/ml. Found in circulating blood plasma complexed with two C1r and two C1s molecules to form the first component of complement (C1). Activation of complement via classical pathway is triggered by binding of C1q to immune complexes containing IgG or IgM or to a variety of other activating substances, including C-reactive protein, retroviruses, and mitochondria. Subsequent to C1q binding, C1r and C1s are converted to proteolytic enzymes that are responsible for continuation of activation via the classical pathway.
Biochem/physiol Actions
≥150,000 C1qH50 units/mg and ≥70% C1q activity in normal human serum on a mg/mg basis
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 300 mM NaCl, 10 mM HEPES, 40% glycerol, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Loos, M., et al. 1980. J. Immunol. 124, 59.
Kolb, W.P., et al. 1979. J. Immunol. 122, 2103.
Kolb, W.P., et al. 1979. J. Immunol. 122, 2103.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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