48-612MAG

MILLIPLEX^® Akt/mTOR Signaling Panel

Premixed Akt/mTOR Total 11-Plex Cell Signaling Panel

• Synonym(s): Magnetic bead assay, Akt mTOR total assay, Akt total protein, PI3K pathway assay, kinase total assay, mTOR total detection, multiplex total ELISA, total protein panel, total signaling proteins
• NACRES: NA.47
• UNSPSC Code: 12161503

Properties

• Product Name: MILLIPLEX^® Akt/mTOR Signaling Panel, Premixed Akt/mTOR Total 11-Plex Cell Signaling Panel
• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• packaging: 1 ea of
• technique(s): multiplexing: suitable
• input: cell lysate
• detection method: fluorometric (Luminex^® xMAP^® technology)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

• Simultaneously detect total expression of 11 proteins in the PI3K/Akt/mTOR signaling pathway
• Includes Akt1, Akt2, Akt3, mTOR, PTEN, GSK3α/β, IRS1, IGF1R, IR, p70S6K, and RPS6
• Magnetic bead-based format ensures reproducibility and efficient processing
• Compatible with cell lysates from cancer, metabolic, and signaling studies
• Ideal for oncology, metabolism, and cell biology research
• Fast, convenient alternative to Western Blotting and immunoprecipitation procedures

General description

This panel targets total expression of key proteins in the PI3K/Akt/mTOR signaling pathway, which regulates cell growth, metabolism, and survival. It includes total levels of Akt isoforms, mTOR, PTEN, GSK3α/β, IRS1, IGF1R, IR, p70S6K, and RPS6. These proteins are frequently altered in cancer, diabetes, and neurodegenerative diseases.

Application

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 11 analytes in human, mouse, or rat cell lysate samples.

Analytes Included: Akt (Total), GSK3α (Total), GSK3β (Total), IGF-1R (Total), IR (total), IRS1 (Total), mTOR (Total), p70S6K (Total), PTEN (Total), RPS6 (Total), TSC2 (Total)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate^™Control Assays).

Safety Information

• pictograms: GHS07
• Storage Class: 10-13 - German Storage Class 10 to 13
• signalword: Danger
• hcodes: H319,H412,EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
• wgk: WGK 2