GAPDH (46-667MAG) is functional only in Assay Buffer 1 when used at significantly lower dilutions, approximately 1 microgram of total protein per well. This specificity is the reason it is designated for use with Buffer 2 only. Most panels necessitate higher protein concentrations, making it impractical to multiplex GAPDH with panels that utilize Assay Buffer 1. When conducting assays with Assay Buffer 1, B-Tubulin MAPMate (46-713MAG) is the recommended alternative.
48-621MAG
MILLIPLEX® DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay
Synonym(s):
Cell Signaling DNA Damage Kit, DNA Damage Bead Panel, Genotoxicity Magnetic Beads
About This Item
Recommended Products
species reactivity
mouse, human, rat
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
storage temp.
2-8°C
General description
Specificity
Application
Storage and Stability
Legal Information
Disclaimer
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
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Is it possible to incorporate GAPDH as a loading control into the DNA Damage panel (48-621MAG) even though it specifies the use of Assay Buffer 1 and the GAPDH MAPmate (46-667MAG) requires Assay Buffer 2? Does the difference in assay buffer formulations affect the compatibility of adding GAPDH to the panel?
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