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654245

Sigma-Aldrich

TNF-α, Mouse, Recombinant, E. coli

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Synonym(s):
rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α

assay

≥97% (SDS-PAGE)

Quality Level

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤100 pg/μg Endotoxin (pg/μg TNF-α)

shipped in

ambient

storage temp.

−70°C

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This Item
SRP3177407654407617
TNF-α human Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

SRP3177

TNF-α human

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage condition

OK to freeze

storage condition

-

storage condition

OK to freeze

storage condition

OK to freeze

impurities

≤100 pg/μg Endotoxin (pg/μg TNF-α)

impurities

≤1.00 EU/μg endotoxin

impurities

≤1 EU/μg Endotoxin (EU/μg IL-6)

impurities

≤1.0 EU/μg Endotoxin (EU/μg IL-1β)

shipped in

ambient

shipped in

wet ice

shipped in

ambient

shipped in

ambient

General description

Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. It is referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune system′s response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. Referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune systems response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.

Biochem/physiol Actions

ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Other Notes

Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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