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Overnight Express Autoinduction System 1 - Novagen

The Overnight Express Autoinduction System allows the induction of protein expression without monitoring cell density and without conventional induction with IPTG.

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Synonym(s):
autoinduction media, autoinduction medium

Quality Level

200

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage temp.

10-30°C

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71759-M71757-M70837
Overnight Express Autoinduction System 1 - Novagen The Overnight Express Autoinduction System allows the induction of protein expression without monitoring cell density and without conventional induction with IPTG.

Sigma-Aldrich

71300-M

Overnight Express Autoinduction System 1 - Novagen

Sigma-Aldrich

Sigma-Aldrich

71759-M

Overnight Express™ Autoinduction NMR Medium - 15N - Novagen

Overnight Express Instant LB Medium - Novagen

Sigma-Aldrich

71757-M

Overnight Express Instant LB Medium - Novagen

Origami B(DE3) Competent Cells - Novagen Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

Sigma-Aldrich

70837

Origami B(DE3) Competent Cells - Novagen

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage temp.

10-30°C

storage temp.

-

storage temp.

10-30°C

storage temp.

−70°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

form

liquid

form

-

form

solid

form

-

General description

The Overnight Express™ Autoinduction System allows the induction of protein expression without monitoring cell density and without conventional induction with isopropyl β-D-1-thiogalactopyranoside (IPTG). This system is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones. The Overnight Express Autoinduction System together with the Novagen RoboPop Solubility Screening Kit and RoboPop Purification Kits allows rapid optimization of the host-vector combinations, expression conditions, and purification parameters for high-throughput production of proteins for structural or functional analysis.

Application

Overnight Express™ Autoinduction System 1 - Novagen has been used for the purification of 15N-human prion proteins (HuPrPs). It is suitable for most routine recombinant protein expression applications.

Features and Benefits

  • No need to monitor cell growth or add IPTG
  • High cell densities and protein expression levels
  • Increased soluble protein yield
  • Induction of numerous expression clones simultaneously
  • Usable with any conventional glucose-free bacterial growth medium
  • Ideal for pET Expression System or other IPTG-inducible bacterial systems
  • Compatible with cultures grown in flasks, culture tubes, and deep-well plates
  • Minimal sample handling

Components

The Overnight Express™ Autoinduction System 1 kit contains three concentrated sterile solutions:
  • OnEx Solution 1 A blend of carbon sources optimized for tightly regulated uninduced growth to high cell density, followed by high-level induction
  • OnEx Solution 2 A concentrated buffer and nitrogen-source bend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
  • OnEx Solution 3 Provides magnesium for maximal cell density

Warning

Toxicity: Standard Handling (A)

Other Notes

Grabski, A., et al. 2003. inNovations 17, 3.

Legal Information

Express is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

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Andrew G Reidenbach et al.
The Journal of biological chemistry, 295(39), 13516-13531 (2020-07-30)
Prion disease is a rapidly progressive neurodegenerative disorder caused by misfolding and aggregation of the prion protein (PrP), and there are currently no therapeutic options. PrP ligands could theoretically antagonize prion formation by protecting the native protein from misfolding or

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