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AB1766

Sigma-Aldrich

Anti-Dopamine Transporter Antibody, CT

Chemicon®, from rabbit

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Synonym(s):
DAT
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

monkey, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC6A3(6531)

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AB5802AB1591PMAB369
antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

culture supernatant

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

DAT-Nt, monoclonal

species reactivity

monkey, human

species reactivity

human, monkey

species reactivity

rat, mouse

species reactivity

rat, human, mouse, monkey

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

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Specificity

Dopamine Transporter (DAT), C-terminal.

Immunogen

Synthetic peptide from the intracellular C-terminal region of human dopamine transporter (CEKDRELVDRGEVRQFTLRHWL).

Application

Anti-Dopamine Transporter Antibody, C-terminus detects level of Dopamine Transporter & has been published & validated for use in IH & WB.
Immunoblotting: 0.1-1.0 μg/mL. The antibody recognizes the ~88 kDa DAT protein in Western blot of human strial samples.
Immunohistochemistry using formaldehyde-fixed brain sections.
Not recommended for use on rodent.
Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in 100 μL of 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Storage and Stability

Maintain at -20 to -80°C in undiluted aliquots for up to six months after date of receipt. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Zuzanna Kurowska et al.
Journal of Parkinson's disease, 1(1), 83-92 (2011-01-01)
We demonstrate that grafted human fetal mesencephalic neurons can survive and extend axons for 22 years in the brain of a patient with Parkinson's disease (PD). In this patient, the overall survival and fiber outgrowth of the grafts were, however
Chiharu Sogawa et al.
PloS one, 5(8), e11945-e11945 (2010-08-12)
The transporters for dopamine (DAT) and norepinephrine (NET) are members of the Na+- and Cl--dependent neurotransmitter transporter family SLC6. There is a line of evidence that alternative splicing results in several isoforms of neurotransmitter transporters including NET. However, its relevance
Maged M Harraz et al.
Molecular psychiatry, 26(2), 370-382 (2021-01-09)
Cocaine exerts its stimulant effect by inhibiting dopamine reuptake leading to increased dopamine signaling. This action is thought to reflect binding of cocaine to the dopamine transporter (DAT) to inhibit its function. However, cocaine is a relatively weak inhibitor of
Joanne Ng et al.
Science translational medicine, 13(594) (2021-05-21)
Most inherited neurodegenerative disorders are incurable, and often only palliative treatment is available. Precision medicine has great potential to address this unmet clinical need. We explored this paradigm in dopamine transporter deficiency syndrome (DTDS), caused by biallelic loss-of-function mutations in

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