IEVH85R
Immobilon® -E PVDF Membrane
1 roll, 8.5 cm × 10 m, 0.45 µm pore size, transfer membrane wets out in transfer buffer or water
Synonym(s):
Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane
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Product Name
Immobilon®-E PVDF Membrane, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
material
PVDF membrane
plain filter
white filter
Quality Level
feature
easily wetted with water
manufacturer/tradename
Immobilon®
technique(s)
western blot: suitable
filter L × W
8.5 cm × 10 m
pore size
0.45 μm pore size
compatibility
for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination
detection method
chemiluminescent
colorimetric
radioactive
shipped in
ambient
1 of 4
This Item | IEVH07850 | IEVH00005 | IEVH09120 |
|---|---|---|---|
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| compatibility for use with Amido black, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Transillumination, for use with Colloidal gold, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with India ink | compatibility for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination | compatibility for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination | compatibility for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination |
| detection method colorimetric, radioactive, chemiluminescent | detection method chemiluminescent, colorimetric, radioactive | detection method chemiluminescent, colorimetric, radioactive | detection method chemiluminescent, colorimetric, radioactive |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
General description
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.
Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
Legal Information
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
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Articles
Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Protocols
Resumen de la química del gel de poliacrilamida e instrucciones detalladas para preparar a mano geles de poliacrilamida con los kits de moldeo del gel bis-Tris TurboMix mPAGE®
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Related Content
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.
Lo entendemos. Los Westerns pueden ser un dolor de muelas, y ciertamente hemos hecho nuestra parte. Así que, ¡comparte con nosotros tus feos borrones y recibe una sorpresa! Y aunque no podemos decirte de qué se trata -eso estropearía la sorpresa-, salvo que despertará la creatividad en el laboratorio.
Protein Blotting Survival Handbook: Tips and Tricks
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
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