MAB5270

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, mouse monoclonal, 1.B3.9B3

• Synonym(s): ChAT
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• Product Name: Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3, clone 1.B3.9B3, Chemicon^®, from mouse
• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1.B3.9B3, monoclonal
• species reactivity: human, rat, pig
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_020549.3; NM_020984.2; NM_020985.2; NM_020986.2
• UniProt accession no.: P28329
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CHAT(1103) ; pig ... Chat(396896) ; rat ... Chat(290567)

Description

General description

69 kDa

Immunogen

Highly purified ChAT obtained from porcine brain (Ostermann-Fatif et al., 1990).

Application

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 is validated for use in ELISA, IH, IH(P), WB for the detection of Choline Acetyltransferase.

Western blot

ELISA

Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}

Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Reacts with choline acetyltransferase (ChAT)-positive cells from humans, rats and pigs (Ostermann-Fatif et al., 1992a,b).

Physical form

Format: Purified

Protein A purified

Purified immunoglobulin. Lyophilized from 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. Reconstitute to 100 μg/mL with sterile distilled water.

Preparation Note

Maintain lyophilized antibody at 2–8°C in undiluted aliquots for up to 6 months. Maintain reconstituted antibody at -20°C in undiluted aliquots for up to 6 months.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.