MAB5350

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, mouse monoclonal, 28C4

• Synonym(s): ChAT
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• Product Name: Anti-Choline Acetyltransferase Antibody, clone 28C4, clone 28C4, Chemicon^®, from mouse
• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 28C4, monoclonal
• species reactivity: human, rat, guinea pig, monkey
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_020549.3; NM_020984.2; NM_020985.2; NM_020986.2
• UniProt accession no.: P28329
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CHAT(1103) ; rat ... Chat(290567) ; rhesus monkey ... Chat(709977)

Description

Immunogen

Purified human placenta ChAT.

Application

Anti-Choline Acetyltransferase Antibody, clone 28C4 detects level of Choline Acetyltransferase & has been published & validated for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Western blotting: 1:1,000-1:10,000 using a high sensitivity ECL kit. Recognizes a protein of ~67 kDa. May also recognize a protein of 35 kDa which is thought to be a degradation product. Suggested dilution buffer is TBS containing 5% skim milk powder and 0.05% Tween 20.

Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.

ELISA (two side)

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with Choline Acetyltransferase (ChAT).

Physical form

Format: Purified

Purified immunoglobulin. Liquid.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable