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PIHP01250

Millipore

Millicell Cell Culture Insert, 12 mm, polycarbonate, 0.4 µm

Polycarbonate cell culture insert with pore size of 0.4 µm used in a 24-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.

Synonym(s):
tissue culture plate, tissue culture insert, permeable culture insert, cell culture inserts
eCl@ss:
32011202
NACRES:
NB.22

material

flat wells
polycarbonate membrane
polystyrene

Quality Level

sterility

sterile

feature

lid: no

packaging

pack of 48

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

application(s)

cell analysis: suitable (cell attachment)
cell analysis: suitable (cell differentiation)
cell culture | mammalian: suitable
drug transporter assay: suitable
permeability assay: suitable

H

10.5 mm

filter diam.

12 mm

filtration area

0.6 cm2

plate size

1 wells

working volume

400 μL

matrix

Isopore

pore size

0.4 μm pore size

input

sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type cardiac stem cell(s)

surface area

0.6 cm2

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

General description

Device Configuration: 24-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
Isopore Membrane (polycarbonate)
- For growth of attachment-dependent cells without matrix

Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry

Packaging

50 pk in Individually blister packaged

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
Peiying Tian et al.
Cell biology international, 44(8), 1681-1690 (2020-04-14)
Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The...
Susanne Elisabeth Pors et al.
Molecular human reproduction, 26(5), 301-311 (2020-03-24)
In vitro activation of resting ovarian follicles, with the use of mechanical stress and/or pharmacological compounds, is an emerging and novel approach for infertility treatment. The aim of this study was to assess the sphingolipid, sphingosine-1-phosphate (S1P), as a potential...
Protocols
This is a Toluidine Blue Staining protocol listing materials and methods.
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This protocol covers 3 modes for the microscopic examination of cell samples.
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This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
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This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
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Related Content
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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