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UFC30GV0S

Millipore

Ultrafree® Centrifugal Filter, 0.5 mL Sample Volume

pore size 0.22 μm, PVDF membrane (hydrophilic)

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Synonym(s):
Centrifuge filter
eCl@ss:
36100101
NACRES:
NB.24

material

PVDF membrane (hydrophilic)
polypropylene

Quality Level

400

sterility

sterile

feature

holdup volume 5 μL

manufacturer/tradename

Ultrafree®

parameter

0.5 mL sample volume
50 °C max. temp.

technique(s)

protein extraction: suitable
protein purification: suitable

L

45 mm

diam.

10.6 mm

filtration area

0.2 cm2

matrix

Durapore®

pore size

0.22 μm pore size

application(s)

sample preparation

shipped in

ambient

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This Item
UFC30VVUFC30DV25UFC40GV0S
Ultrafree®-MC GV Centrifugal Filter 0.22 μm Hydrophilic PVDF, Sterile, 0.5 mL sample volume

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UFC30GV0S

Ultrafree®-MC GV Centrifugal Filter

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UFC30VV

Ultrafree-MC Centrifugal Filter

Ultrafree-MC Centrifugal Filter 0.65 µm pore size, hydrophilic PVDF, 0.5 mL volume, non-sterile

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UFC30DV25

Ultrafree-MC Centrifugal Filter

Ultrafree-CL Centrifugal Filter 0.22 µm pore size, hydrophilic PVDF, 2 mL volume, sterile

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UFC40GV0S

Ultrafree-CL Centrifugal Filter

sterility

sterile

sterility

non-sterile

sterility

non-sterile

sterility

sterile

manufacturer/tradename

Ultrafree®

manufacturer/tradename

Ultrafree®

manufacturer/tradename

Ultrafree®

manufacturer/tradename

Ultrafree®

pore size

0.22 μm pore size

pore size

0.1 μm pore size

pore size

0.65 μm pore size

pore size

0.22 μm pore size

feature

holdup volume 5 μL

feature

holdup volume 5 μL

feature

holdup volume 5 μL

feature

holdup volume 10 μL

parameter

0.5 mL sample volume

parameter

0.5 mL sample volume, 50 °C max. temp.

parameter

0.5 mL sample volume, 50 °C max. temp.

parameter

2 mL sample volume, 50 °C max. temp.

General description

Ultrafree®-MC (0.5 mL) and Ultrafree®-CL (2 mL) centrifugal devices are single-use, disposable filters used for removing particulates from aqueous biological solutions. These devices are available in two processing volumes with a range of microporous membranes (from 0.1 to 5.0 m) for fast filtration and highly reproducible performance. Pre-sterilized units are also available. Use in fixed-angle rotors for 1.5 mL tubes (-MC) and 15 mL tubes (CL).

Filter Code: GVPP
Filter Type: Microfiltration
Ultrafree®-MC (0.5 mL) and Ultrafree®-CL (2 mL) centrifugal devices are single-use, disposable filters used for removing particulates from aqueous biological solutions. These devices are available in two processing volumes with a range of microporous membranes (from 0.1 to 5.0 m) for fast filtration and highly reproducible performance. Pre-sterilized units are also available. Use in fixed-angle rotors for 1.5 mL tubes (-MC) and 15 mL tubes (CL).
Filter Code: GVPP
Filter Type: Microfiltration

Application

Ultrafree®-MC GV Centrifugal Filter has been used


  • to filter cell lysates after centrifugation for Label-free mass spectrometry
  • for filtering extracted enzymes in the buffer for preparing imaging solution for cellular direct stochastic optical reconstruction microscopy (dSTORM) and DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) imaging
  • to purify viral solution during transfection studies
  • to filter heart tissue homogenates for preparation of the sample for high-pressure liquid chromatography (HPLC) analysis

Features and Benefits

  • Used for removal of particles and precipitates from aqueous and some solvent-based samples
  • Ideal for use with protein and nucleic acid solutions
  • Used for removal of particles and precipitates from aqueous and some solvent-based samples
  • Ideal for use with protein and nucleic acid solutions

Physical form

Color Code: Yellow

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany

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Cetin Agar et al.
Blood, 117(25), 6939-6947 (2011-04-02)
Sepsis is a systemic host response to invasive infection by bacteria. Despite treatment with antibiotics, current mortality rates are in the range of 20%-25%, which makes sepsis the most important cause of death in intensive care. Gram-negative bacteria are a
Robert Player et al.
BMC genomics, 21(1), 166-166 (2020-02-19)
The state-of-the-art in nucleic acid based biodetection continues to be polymerase chain reaction (PCR), and many real-time PCR assays targeting biodefense pathogens for biosurveillance are in widespread use. These assays are predominantly singleplex; i.e. one assay tests for the presence
Beta2-glycoprotein I can exist in 2 conformations: implications for our understanding of the antiphospholipid syndrome.
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Protocols

Affinity Purification with Ultrafree®-MC Centrifugal Filters

Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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