This kit is used for the detection of hippurate hydrolase, an enzyme produced by a number of pathogenic organisms. Hippurate is the substrate embedded into the test strips. Hippurate hydrolase produced by bacteria will hydrolyze the embedded hippurate in the test strip. One of the resulting by-products, glycine, reacts with the chromogen producing a blue/purple color, indicating a positive test. This is a qualitative test. Tests are limited to cultures taken from samples that have been incubated for at least 24 hours. See the link below to review the protocol: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/398/494/01869dat.pdf
01869
Hippurate Strips Kit
suitable for microbiology
Synonym(s):
Hippurate Strips
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1 KIT
$57.00
About This Item
UNSPSC Code:
41171621
NACRES:
NA.85
Recommended Products
technique(s)
microbe id | specific enzyme detection: suitable
Quality Level
application(s)
clinical testing
environmental
food and beverages
microbiology
storage temp.
2-8°C
suitability
Campylobacter spp.
Gardnerella vaginalis
Streptococcus spp.
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Application
Test for identification of hippurate hydrolase activity of group B streptococci, Campylobacter jejuni, Gardnerella vaginalis and other microorganisms.
The test is based on hydrolysis of substrate (sodium hippurate) by the bacterial enzyme hippurate hydrolase and production of benzoic acid and glycine. Glycine produced by this enzymatic reaction is detected after 24 hours incubation in reaction with chromogen (ninhydrine) and the blue-purple substance is produced.
The test is based on hydrolysis of substrate (sodium hippurate) by the bacterial enzyme hippurate hydrolase and production of benzoic acid and glycine. Glycine produced by this enzymatic reaction is detected after 24 hours incubation in reaction with chromogen (ninhydrine) and the blue-purple substance is produced.
Components
The kit contains 50 Hippurate Strips (Cat. No. 92472) saturated with sodium hippurate and 350 mg Chromogen in 15 ml Diluent (Cat. No. 01875)
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
50.0 °F - closed cup
flash_point_c
10 °C - closed cup
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
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Hello, Is this product suitable to detect if there is any hippurate in our samples? Is it a quantitative test or a qualitative test? Are there any restrictions on sample types?
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