Mueller Hinton Agar

suitable for microbiology, NutriSelect® Plus


Quality Level



product line




shelf life

limited shelf life, expiry date on the label


agar, 17.0 g/L
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L


NutriSelect® Plus


microbe id | susceptibility testing: suitable
microbiological culture: suitable
microbiology: suitable

final pH

7.3±0.2 (25 °C)

Featured Industry

Clinical Testing
Food and Beverages


nonselective for Neisseria spp.
nonselective for bacteria (General Media)

Related Categories


Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.
Mueller Hinton Agar has been used:
  • in culturing Escherichia coli ATCC® 25922 strain for disc diffusion studies
  • in disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
  • in agar diffusion assay of drug loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli

Preparation Note

Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar

Legal Information

ATCC is a registered trademark of American Type Culture Collection
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Multi-drug delivery system based on lipid membrane mimetic coated nano-hydroxyapatite formulations
Placente D, et al.
International Journal of Pharmaceutics, 548(1), 559-570 (2018)
G M Eliopoulos et al.
Diagnostic microbiology and infectious disease, 12(2), 149-156 (1989-03-01)
The activities of cefotaxime and other aminothiazoyl oxime cephalosporins against Enterococcus faecalis were enhanced by addition of 5% sheep blood to Mueller-Hinton agar. This effect was not seen with aztreonam (aminothiazoyl oxime monobactam), cefotiam (aminothiazoyl, nonoxime), or other cephalosporins, and...
Preparation and evaluation of QbD based fusidic acid loaded in situ gel formulations for burn wound treatment
Aksu NB, et al.
Journal of drug delivery science and technology, 52, 110-121 (2019)
R D Jenkins et al.
Journal of clinical microbiology, 22(3), 369-374 (1985-09-01)
Disk diffusion susceptibility tests for enterococci are frequently modified by adding 5% sheep blood (SB) to Mueller-Hinton agar; the performance standards from the National Committee for Clinical Laboratory Standards sanction this addition. Susceptibility testing of aminoglycoside antibiotics is not recommended...
Solid-state structure and antimicrobial and cytotoxicity studies of a cucurbit [6] uril-like Cu6L4 constructed from 3, 5-bis [(1H-tetrazol-5-yl) methyl]-4H-1, 2, 4-triazol-4-amine
Vasylevskyi S, et al.
Acta Crystallographica Section C, Structural Chemistry, 74(11) (2018)
Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.
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Self-assembled monolayers (SAMs) have attracted enormous interest for a wide variety of applications in micro- and nano-technology. In this article, we compare the benefits of three different classes of SAM systems (alkylthiolates on gold).
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