77608

Phenol

BioUltra, Molecular Biology, ≥99.5% (GC)

• Synonym(s): Hydroxybenzene
• Linear Formula: C₆H₅OH
• CAS Number: 108-95-2
• Molecular Weight: 94.11
• UNSPSC Code: 12352104
• NACRES: NA.25
• PubChem Substance ID: 329767806
• EC Number: 203-632-7
• Beilstein/REAXYS Number: 969616
• MDL number: MFCD00002143

Properties

• Product Name: Phenol, BioUltra, Molecular Biology, ≥99.5% (GC)
• cation traces: Sr: ≤5 mg/kg; Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• SMILES string: Oc1ccccc1
• grade: Molecular Biology
• vapor density: 3.24 (vs air)
• vapor pressure: 0.09 psi ( 55 °C); 0.36 mmHg ( 20 °C)
• product line: BioUltra
• assay: ≥99.5% (GC)
• form: solid
• autoignition temp.: 1319 °F
• contains: ~0.15% hypophosphorous acid as stabilizer
• expl. lim.: 8.6 %
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.5% water
• ign. residue: ≤0.05%
• pH: 3.0-6.0 (25 °C, 0.5 M in H₂O)
• bp: 182 °C (lit.)
• mp: 40-42 °C (lit.); 40-42 °C
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• density: 1.071 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• absorption: cut-off at 294 nm in H₂O at 0.5 M
• suitability: suitable for molecular biology
• storage temp.: 2-8°C
• Quality Level: 100

Description

Application

Phenol has been used in the incubation of pellet for RNA extraction.[1]

Biochem/physiol Actions

Phenol has the ability to denature protein, hence can lead to denervation.[2] At lower concentration, it can serve as a local anaesthetic and can also act as a neurolytic agent in higher concentration.[3] It is also linked with tissue damage at higher concentrations.[2]

General description

Phenol/carbolic acid[2] is mainly produced by hospital pharmacy as it is unavailable in premixed liquid form. It is not highly soluble in water. Phenol should be refrigerated and protected from light exposure to maintain its shelf life of approximately one year.[4] Kidneys can excrete phenol as several conjugated derivatives.[3]

Other Notes

For the preparation of DNA [5]; Large- and small-scale phenol extractions[6]

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H311 + H331,H314,H341,H373,H411
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• target_organs: Nervous system,Kidney,Liver,Skin
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 2
• flash_point_f: 174.2 °F - closed cup
• flash_point_c: 79.0 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges