77608

Phenol

BioUltra, Molecular Biology, ≥99.5% (GC)

• Synonym(s): Hydroxybenzene
• Linear Formula: C₆H₅OH
• CAS Number: 108-95-2
• Molecular Weight: 94.11
• UNSPSC Code: 12352104
• NACRES: NA.25
• PubChem Substance ID: 329767806
• EC Number: 203-632-7
• Beilstein/REAXYS Number: 969616
• MDL number: MFCD00002143
• Assay: ≥99.5% (GC)
• Form: solid

Properties

• grade: Molecular Biology
• Quality Segment: 100
• vapor density: 3.24 (vs air)
• vapor pressure: 0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)
• product line: BioUltra
• assay: ≥99.5% (GC)
• form: solid
• autoignition temp.: 1319 °F
• contains: ~0.15% hypophosphorous acid as stabilizer
• expl. lim.: 8.6 %
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.5% water
• ign. residue: ≤0.05%
• pH: 3.0-6.0 (25 °C, 0.5 M in H₂O)
• bp: 182 °C (lit.)
• mp: 40-42 °C
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• density: 1.071 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg
• SMILES string: Oc1ccccc1
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• absorption: cut-off at 294 nm in H₂O at 0.5 M
• suitability: suitable for molecular biology
• storage temp.: 2-8°C
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Description

General description

Phenol/carbolic acid is mainly produced by hospital pharmacy as it is unavailable in premixed liquid form. It is not highly soluble in water. Phenol should be refrigerated and protected from light exposure to maintain its shelf life of approximately one year. Kidneys can excrete phenol as several conjugated derivatives.

Application

Phenol has been used in the incubation of pellet for RNA extraction.

Biochem/physiol Actions

Phenol has the ability to denature protein, hence can lead to denervation. At lower concentration, it can serve as a local anaesthetic and can also act as a neurolytic agent in higher concentration. It is also linked with tissue damage at higher concentrations.

Other Notes

For the preparation of DNA ; Large- and small-scale phenol extractions

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Codes: H301 + H311 + H331,H314,H341,H373,H411
• Precautionary Statements: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• Target Organs: Nervous system,Kidney,Liver,Skin
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 2
• Flash Point (°F): 174.2 °F - closed cup
• Flash Point (°C): 79.0 °C - closed cup
• PPE (personal protective equipment): Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges