A5306

Sigma-Aldrich

Bacteriological agar

for molecular biology

Synonym(s):
Agar-agar, Agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
NACRES:
NA.31

Quality Level

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General description

Bacteriological agar is a colloidal substance and a cell wall component, obtained from Rhodoyceae (marine algae) and Gelidium sp. Agarose combined with agaropectin forms this polysaccharide. It is also called as agar-agar and is a polymer of galactoside. This agar is soluble in boiling water but not in cold water.

Application

Bacteriological agar has been used:
  • as a component of ATCC agar for Escherichia coli culture
  • as one of the experimental diet feed for Atlantic salmon fry, to study the protein and lysine requirements for maintenance and for tissue accretion
  • as component of yeast extract peptone dextrose medium, Luria broth medium and synthetic complete drop out medium for Escherichia coli culture

Packaging

1 kg in poly bottle

Biochem/physiol Actions

Bacteriological agar is commonly used as a culture medium for microorganism. It is useful for fermentation process. Agar-agar serves as a preservative in food processing. It also possesses various other applications such as an emulsifier, carrier, lubricant, stabilizer, laxative disintegrant in pharmaceutical and cosmetic industries. Agar-agar is also used in photographic emulsion.

Quality

A purified agar in which the original naturally occurring pigments, salts and miscellaneous matter have been reduced to a minimum.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Protein and lysine requirements for maintenance and for tissue accretion in Atlantic salmon (Salmo salar) fry
Abboudi T, et al.
Aquaculture (Amsterdam, Netherlands), 261(1), 369-383 (2006)
Decontamination of materials contaminated with Francisella philomiragia or MS 2 bacteriophage using PES-Solid, a solid source of peracetic acid
Buhr TL, et al.
Journal of Applied Microbiology, 117(2), 397-404 (2014)
Measuring the activity of protein variants on a large scale using deep mutational scanning
Fowler DM, et al.
Nature Protocols, 9(9), 2267-2267 (2014)
Handbook of Fillers, Extenders, and Diluents, 223-223 (2007)
Natural Food Antimicrobial Systems, 417-417 (2000)
Protocols
Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.
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