M6880

Malachite Green oxalate salt

Powder, crystals or chunks

• Synonym(s): N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate, Basic Green 4
• Empirical Formula (Hill Notation): C₂₃H₂₅N₂ · C₂HO₄ · 0.5C₂H₂O₄
• CAS Number: 2437-29-8
• Molecular Weight: 463.50
• Colour Index Number: 42000
• Beilstein/REAXYS Number: 3644550
• EC Number: 219-441-7
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897123
• NACRES: NA.47

Properties

• Product Name: Malachite Green oxalate salt, Technical grade
• grade: technical grade
• Quality Level: 200
• form: powder, crystals or chunks
• technique(s): microbe id | staining: suitable
• color: metallic green
• solubility: water: 1 mg/mL
• λmax: 616-620 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O
• InChI: 1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1
• InChI key: REZFCYOVFCJTDG-UHFFFAOYSA-M

Description

General description

The release of phosphate or pyrophosphate in an enzymatic reaction can be detected by malachite green and is measured spectrometrically at 660nm.[1]

Application

Malachite Green oxalate salt has been used in biliary phospholipid analysis.[2]

Malachite Green oxalate salt has been used:

• in the preparation of malachite green-ammonium molybdate reagent, as a stop solution for starch synthase activity assay[3]
• to terminate the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) inhibition assay using phosphatidylinositol (3,4,5) triphosphate (PI(3,4,5)P3) as substrate[4]
• to determine trypanothione synthetase activity to measure inorganic phosphate release from adenosine triphosphate (ATP) during catalysis[5]

Biochem/physiol Actions

Malachite green is used in the treatment of fungal and parasitic infections in fish.[5]

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301,H318,H361fd,H410
• pcodes: P202 - P264 - P273 - P280 - P301 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable