M9015

Sigma-Aldrich

Malachite Green oxalate salt

certified by the Biological Stain Commission

Synonym(s):
Basic Green 4, N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate
Empirical Formula (Hill Notation):
C23H25N2 · C2HO4 · 0.5C2H2O4
CAS Number:
Molecular Weight:
463.50
Colour Index Number:
42000
Beilstein/REAXYS Number:
3644550
EC Number:
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.

Quality Level

grade

certified by the Biological Stain Commission

form

powder or crystals

composition

Dye content, ≥90%

application(s)

hematology: suitable
histology: suitable

mp

144-150 °C

solubility

H2O: 1 mg/mL, blue to very deep blue

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O

InChI

1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1

InChI key

REZFCYOVFCJTDG-UHFFFAOYSA-M

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General description

Malachite Green is a basic dye belonging to the triphenylmethane group. It is required in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.

Application

Malachite Green oxalate salt has been used:
  • in the preparation of Alexander staining solution for cell viability test
  • in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)
  • as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method

Malachite Green oxalate salt has been used for phosphate quantification.

Packaging

25, 100 g in glass bottle

Suitability

Certified for use as a counterstain for paraffin embedded botanical material; as a spore stain for bacteria by the method of Schaeffer and Fulton.

Signal Word

Danger

RIDADR

UN2811 - class 6.1 - PG 3 - EHS - Toxic solids, organic, n.o.s., HI: all

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects.
Papp D, et. al.
Autophagy, 12, 273-273 (2016)
Biophysical analysis of the effect of chemical modification by 4-oxononenal on the structure, stability, and function of binding immunoglobulin protein (BiP)
Shah DD, et al.
Testing, 12(9), e0183975-e0183975 (2017)
Synthetic Dyes in Biology, Medicine And Chemistry (2012)
Rapid separation of Arabidopsis male gametophyte developmental stages using a Percoll gradient
Duplakova N, et al.
Nature Protocols, 11(10), 1817-1817 (2016)
Methods in Plant Cell Biology (1995)

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