STS0200

ECO TWEEN^® 20

viscous liquid

• Synonym(s): TWEEN^® 20, Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
• CAS Number: 9005-64-5
• MDL number: MFCD00165986

Properties

• description: non-ionic
• Quality Level: 200
• Assay: ≥40.0% (GC)
• form: viscous liquid
• mol wt: ~1228
• composition: lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
• greener alternative product characteristics: Use of Renewable Feedstocks; Design for Degradation; Learn more about the Principles of Green Chemistry.
• impurities: ≤3.0% water
• refractive index: n20/D 1.468 (lit.)
• CMC: 0.06 mM (20-25°C)
• acid number: ≤2.2 mg KOH/g
• hydroxyl value: 96‑108 mg KOH/g
• transition temp: cloud point 76 °C
• density: 1.095 g/mL at 25 °C (lit.)
• HLB: 16.7
• greener alternative category: Aligned
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• InChI key: HMFKFHLTUCJZJO-UHFFFAOYSA-N

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is a biobased surfactant and is aligned with the 7th principle of Green Chemistry "Use of Renewable Feedstocks" and the 10th principle "Design for Degradation".

TWEEN 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 has been used as a component of:

• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry
• washing buffer in ELISA (enzyme linked immunosorbent assay)
• reaction mixture in PCR (polymerase chain reaction)

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Non-ionic detergent

Features and Benefits

• 100 % Renewable
• 100 % Bio-based
• Certified to the USDA BioPreferred Program
• Lower carbon footprint than petrochemical-based versions

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 527.0 °F - Pensky-Martens closed cup
• Flash Point(C): 275 °C - Pensky-Martens closed cup