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71736

Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, Molecular Biology, 10% in H2O

Synonym(s):

Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution

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100 ML
$78.60
500 ML
$195.00

About This Item

Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
151-21-3
Molecular Weight:
288.38
Beilstein/REAXYS Number:
3599286
MDL number:
MFCD00036175
UNSPSC Code:
12161902
eCl@ss:
39093306
PubChem Substance ID:
57652355
NACRES:
NA.26

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grade

Molecular Biology

description

anionic

product line

BioUltra

form

liquid

mol wt

288.38 g/mol

concentration

10% in H2O

technique(s)

HPLC: suitable
protein quantification: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

5.0-6.5

density

1.01 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg
phosphate (PO43-): ≤1 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.3
λ: 280 nm Amax: 0.2

application(s)

(Western Blot)

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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42801871725L3771
Sodium dodecyl sulfate solution BioUltra, Molecular Biology, 10% in H2O

71736

Sodium dodecyl sulfate solution

Sodium dodecyl sulfate solution 20% Solution (w/v), An aqueous solution containing 20% SDS.

428018

Sodium dodecyl sulfate solution

Sodium dodecyl sulfate BioUltra, Molecular Biology, ≥99.0% (GC)

71725

Sodium dodecyl sulfate

Sodium dodecyl sulfate BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)

L3771

Sodium dodecyl sulfate

grade

for molecular biology

grade

-

grade

for molecular biology

grade

for molecular biology

form

liquid

form

liquid

form

powder

form

powder

technique(s)

HPLC: suitable, protein quantification: suitable

technique(s)

-

technique(s)

-

technique(s)

electrophoresis: suitable

impurities

DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, phosphatases, none detected

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

≤2.0% water (Karl Fischer)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Ca: ≤5 mg/kg, Co: ≤1 mg/kg, Cu: ≤1 mg/kg, K: ≤20 mg/kg, Mg: ≤1 mg/kg, Mo: ≤1 mg/kg, Pb: ≤1 mg/kg, Zn: ≤1 mg/kg, As: ≤0.1 mg/kg, Cd: ≤1 mg/kg, Fe: ≤1 mg/kg, Mn: ≤1 mg/kg, Sr: ≤1 mg/kg, Bi: ≤1 mg/kg, Li: ≤1 mg/kg, Cr: ≤1 mg/kg, Ni: ≤1 mg/kg

cation traces

-

cation traces

Al: ≤5 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤200 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

cation traces

heavy metals (as Pb): ≤10 ppm

density

1.01 g/mL at 20 °C

density

-

density

0.455 g/cm3

density

1.03 g/cm3 at 20 °C (68 °F)

Application

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer[1]
  • as a component in elution buffer for immunoprecipitation[2]
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis[3][4]

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids.[5] It is also considered as a permeability enhancer in oral formulations.[6]

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material[7]

related product

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Description
Pricing

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1388
BCCM9637
BCCM3869
BCCL6561
BCCL3786

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