71736

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H₂O

• Synonym(s): Sodium lauryl sulfate solution, Lauryl sulfate sodium salt, SDS
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein/REAXYS Number: 3599286
• MDL number: MFCD00036175
• eCl@ss: 39093306
• PubChem Substance ID: 57652355
• NACRES: NA.26

Properties

• Quality Level: 200
• grade: for molecular biology
• description: anionic
• product line: BioUltra
• mol wt: 288.38 g/mol
• concentration: 10% in H₂O
• application(s): HPLC: suitable; protein quantification: suitable
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.0-6.5
• density: 1.01 g/mL at 20 °C
• anion traces: chloride (Cl^-): ≤50 mg/kg; phosphate (PO₄^3-): ≤1 mg/kg
• cation traces: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.3; λ: 280 nm A_max: 0.2
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Description

Application

Sodium dodecyl sulfate solution has been used:

• in the preparation of 2x extraction buffer
• as a component in elution buffer for immunoprecipitation
• in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Personal Protective Equipment: dust mask type N95 (US),Eyeshields,Gloves
• RIDADR: NONH for all modes of transport
• WGK Germany: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable