A0561

ACCUSPIN^™ System-Histopaque^®-1077

• MDL number: MFCD01092091
• NACRES: NA.75

Properties

• sterility: aseptically filled
• Quality Level: 400
• shelf life: Expiry date on the label.
• packaging: package of 100 tubes
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit").

γ-irradiated 50 mL polypropylene tubes fitted with a HDPE porous barrier and asepticallyfilled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.

Application

ACCUSPIN System-Histopaque-1077 is used for the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradient separation media. Histopaque 1077 is suitable for human lymphocyte antigen (HLA) typing and as the initial isolation step prior to enumeration of T, B, and ‘null’ lymphocytes. It may also be employed in the preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.

It is also suitable for the purification of human bone marrow aspirates to investigated the therapeutic value of BET inhibitors for acute myeloid leukemia.

ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

Features and Benefits

ACCUSPIN^™ System-Histopaque^®-1077 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Histopaque is sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

Principle

The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves