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A6929

Sigma-Aldrich

ACCUSPIN System-Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

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Select a Size

MDL number:
NACRES:
NA.75

Quality Level

sterility

aseptically filled

form

solution

shelf life

Expiry date on the label.

application(s)

hematology
histology

storage temp.

2-8°C

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This Item
1077110831A2055
Histopaque®-1077 sterile-filtered, density: 1.077 g/mL

Sigma-Aldrich

10771

Histopaque®-1077

Sigma-Aldrich

Sigma-Aldrich

10831

Histopaque®-1083

form

solution

form

-

form

-

form

-

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

200

luminometer, plasma separation, serum separation, molecular separation, separation by molecular weight, extraction and separation, cell separation, mixture separation, tissue separation, thermal cycler, lymphocytes seperation, whole blood seperation,

luminometer, plasma separation, serum separation, molecular separation, separation by molecular weight, extraction and separation, cell separation, mixture separation, tissue separation, thermal cycler, lymphocytes seperation, whole blood seperation,

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General description

ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit").

γ-irradiated 12 mL polypropylene tubes fitted with a HDPE porous barrier and asepticallyfilled with 3 mL of Histopaque 1077. Each tube will separate 3-6 mL of anticoagulated blood.

Application

ACCUSPIN System-Histopaque-1077 is used for the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradient separation media. Histopaque 1077 is suitable for:
  • human lymphocyte antigen (HLA) typing and as the initial isolation step prior to enumeration of T, B, and ‘null’ lymphocytes.
  • the preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
It is also suitable for the purification of human bone marrow aspirates to investigated the therapeutic value of BET inhibitors for acute myeloid leukemia.
ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

Features and Benefits

ACCUSPIN System-Histopaque®-1077 offers numerous advantages, such as:
  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Histopaque is sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, and resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

Quantity

Package contains 40 prefilled tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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