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A7470

Millipore

Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit

affinity isolated antibody

Synonym(s):
Anti-c-Myc
NACRES:
NA.56

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

analyte chemical class(es)

proteins (c-Myc)

application(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

capacity

≥10 nmol/mL binding capacity (c-Myc tagged fusion protein)
≥7 nmol/mL elution capacity (c-Myc tagged fusion protein)

conjugate

agarose conjugate

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... MYC(4609)

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General description

The human c-Myc proto-oncogene belongs to MYC oncogene family and is the human cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses. c-Myc gene is localized on human chromosome 8q24.21. The two major domains of include the N-terminal box regions and the C-terminal basic-region with helix-loop-helix/leucine-zipper motif.

Specificity

Anti-c-Myc recognizes an epitope located on c-Myc tagged fusion proteins.

Immunogen

synthetic peptide of the human p62c-Myc protein.

Application

Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit has been used in:
  • chromatin immunoprecipitation (ChIP)
  • co-immunoprecipitation
  • western blotting
  • immunofluorescence
  • immunoprecipitation

Biochem/physiol Actions

c-Myc proto-oncogene increased expression is observed in a variety of human tumors. Amplification of Myc gene locus is observed in angiosarcomas and translocation is implicated in Burkitt′s lymphoma. c-Myc is a master regulator and targets a wide variety of metabolic events. An epitope located within amino acid residues 410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tagged fusion proteins. Anti-c-Myc agarose is useful in the purification of expressed c-Myc tagged fusion proteins in bacterial lysates or transfected cells.

Physical form

Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Storage and Stability

For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Riko Hatakeyama et al.
Molecular and cellular biology, 30(24), 5598-5607 (2010-10-20)
Endocytosis of nutrient transporters is stimulated under various conditions, such as elevated nutrient availability. In Saccharomyces cerevisiae, endocytosis is triggered by ubiquitination of transporters catalyzed by the E3 ubiquitin ligase Rsp5. However, how the ubiquitination is accelerated under certain conditions...
Daichao Xu et al.
eLife, 4, e10510-e10510 (2015-11-03)
Regulation of ubiquitin-proteasome system (UPS), which controls the turnover of short-lived proteins in eukaryotic cells, is critical in maintaining cellular proteostasis. Here we show that USP14, a major deubiquitinating enzyme that regulates the UPS, is a substrate of Akt, a...
Wei Wei et al.
Journal of virology, 88(1), 583-591 (2013-11-01)
Sterile alpha motif and HD domain-containing protein 1 (SAMHD1) restricts human immunodeficiency virus type 1 (HIV-1) infection in myeloid cells but is inactivated by certain classes of simian immunodeficiency virus (SIV) Vpx proteins. Vpx proteins recruit the DCAF1-CRL4 E3 ubiquitin...
Julia Verena Hartig et al.
The EMBO journal, 28(19), 2932-2944 (2009-08-01)
Colonization of genomes by a new selfish genetic element is detrimental to the host species and must lead to an efficient, repressive response. In vertebrates as well as in Drosophila, piRNAs repress transposons in the germ line, whereas endogenous siRNAs...
Peter Wehner et al.
Development (Cambridge, England), 138(7), 1321-1327 (2011-02-26)
RACK1 is an evolutionarily conserved intracellular adaptor protein that is involved in a wide range of processes including cell adhesion and migration; however, its role in vertebrate development is largely unknown. Here, we identify RACK1 as a novel interaction partner...

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