Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
C2910
Complement C3 from human serum
≥3000 C3H50 units/mg (using C3 deficient serum)
Promotion Available
Sign In to View Organizational & Contract Pricing.
Spend $500 — Save 5%; Spend $800 — Save 10%; Spend $1,600 — Save 22%*
Sign in to check eligibility. Shop Products
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
0.1 mg | Check Cart for Availability | $947.00 |
About This Item
Form:
solution
Assay:
≥85% (SDS-GE)
Biological source:
human
Mol wt:
185 kDa (disulfide-linked heterodimer)
biological source
human
Quality Segment
assay
≥85% (SDS-GE)
form
solution
specific activity
≥3000 C3H50 units/mg (using C3 deficient serum)
mol wt
185 kDa (disulfide-linked heterodimer)
technique(s)
activity assay: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... C3(718)
Application
Complement C3 is important in complement activation through the classical and alternative pathways. Complement C3 has been used in studies of bacterial defense against the complement system. Research on streptococcus infection has shown that Streptococcal pyrogenic exotoxin B (SPE B), a cysteine protease, can prevent phagocytic activity via cleavage of the C3 and impairing the pathways of the compliment system. C3 deficiency can result in recurrent infections and immune complex disorders. Recent research has shown that inherited C3 deficiency can occur either through homozygous mutations or through compound heterozygous mutations,
Biochem/physiol Actions
Complement C3 is the third and most abundant component of the complement pathway. It plays a central role in complement activation, being involved in both the classical and alternative pathways. C3 or its proteolytic fragments mediate many biological functions such as opsonization and anaphylatoxin activities. Pro-C3 is synthesized as a large single chain of 185 kDa and is processed to a disulfide-linked heterodimer consisting of an α-chain (115 kDa) and a β-chain (70 kDa) in the plasma. C3 is cleaved by the C3 convertases in either pathway (C4b2a, C3iBb, C3bBb) to C3b (176 kDa) comprised of the large C-terminal portion of the α-chain and the β-chain. C3b interacts with other complement components to initiate the amplification cascade.
Complement C3 is the third and most abundant component of the complement pathway. It plays a central role in complement activation, being involved in both the classical and alternative pathways. C3 or its proteolytic fragments mediate many biological functions such as opsonization and anaphylatoxin activities. Pro-C3 is synthesized as a large single chain of 185 kDa and is processed to a disulfide-linked heterodimer consisting of an α-chain (115 kDa) and a β-chain (70 kDa) in the plasma. C3 is cleaved by the C3 convertases in either pathway (C4b2a, C3iBb, C3bBb) to C3b (176 kDa) comprised of the large C-terminal portion of the α-chain and the β-chain. C3b interacts with other complement components to initiate the amplification cascade. Studies of C3 function have been facilitated by experimentally induced C3 deficiency in animals by injection of cobra venom factor and more recently with gene ablation technology.
Physical form
Supplied as a solution in PBS, pH 7.2.
Analysis Note
Functionally pure by a hemolytic assay using deficient sera.
Other Notes
View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
1 of 1
This Item | |||
|---|---|---|---|
| description ≥3000 C3H50 units/mg (using C3 deficient serum) | description ≥90% (SDS-PAGE), >100,000 C5H50 units/mg protein | description suitable for radioiodination, RIA, ≥85% (SDS-PAGE), ≥125,000 C8H50 units/mg protein (using C8 deficient serum) | description for complement assays |
| assay ≥85% (SDS-GE) | assay ≥90% (SDS-PAGE) | assay ≥85% (SDS-PAGE) | assay - |
| technique(s) activity assay: suitable | technique(s) activity assay: suitable | technique(s) immunoelectrophoresis: suitable | technique(s) activity assay: suitable |
| biological source human | biological source human serum | biological source human | biological source human |
| form solution | form solution | form solution | form liquid |
| mol wt 185 kDa (disulfide-linked heterodimer) | mol wt - | mol wt - | mol wt - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
-
-
What is the molecular weight of Product C2910, Complement C3 from human serum?
1 answer-
The molecular weight of Product C2910, Complement C3 from human serum, is approximately 185 kDa. It is composed of 2 nonidenticalpolypeptide chains. The A chain is approximately 110 kDa and the B chain is approximately 70 kDa.
Helpful?
-
-
How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
-