D0819

DMEM - high glucose

Name: DMEM - high glucose
Brand: SIGMA
Price: 39.5 USD

StableCell^™, with stable glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM

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500 ML

$39.50

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

Key Documents

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Product Name

StableCell^™ DMEM - high glucose, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

suitability

suitable for

components

stable glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
glucose: high
NaHCO₃: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Dulbecco's Modified Eagle Medium (DMEM)

Molecular Biology & Functional Genomics

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Show Differences

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This Item	D0697	D0822	FG0435
Sigma-Aldrich D0819 StableCell^™ DMEM - high glucose	Sigma-Aldrich D0697 StableCell^™ DMEM/F12	Sigma-Aldrich D0822 StableCell^™ DMEM - high glucose	Sigma-Aldrich FG0435 Dulbecco′s Modified Eagle′s Medium - high glucose
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
suitability suitable for	suitability -	suitability -	suitability -
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components stable glutamine: yes phenol red: yes HEPES: no sodium pyruvate: no glucose: high NaHCO₃: yes	components glucose: high sodium pyruvate: yes stable glutamine: yes NaHCO₃: yes phenol red: yes HEPES: yes	components glucose: high HEPES: no stable glutamine sodium pyruvate: yes phenol red: yes NaHCO₃: yes	components phenol red: yes glucose: high stable glutamine: yes sodium pyruvate: no HEPES: no NaHCO₃: yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

General description

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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