F4641

Sigma-Aldrich

Monoclonal Anti-Human IgG3−FITC antibody produced in mouse

clone HP-6050, purified from hybridoma cell culture

Synonym(s):
Monoclonal Anti-Human IgG3
NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

HP-6050, monoclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

dot immunobinding: 1:64
particle immunofluorescence: 1:8

isotype

IgG1

conjugate

FITC conjugate

shipped in

dry ice

storage temp.

−20°C

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General description

Human Immunoglobulin G consists of four subclasses, namely IgG1, IgG2, IgG3, and IgG4, named γ1, γ2, γ3, and γ4, respectively. The primary structure of the antibody contains disulfide bonds involved in linking the two heavy chains,and linking the heavy and light chains. IgG3, a pro-inflammatory antibody, appears first in the course of a viral infection and induces effector functions.

Specificity

The antibody is specific for human IgG3 and is non-reactive with other IgG subclasses. This clone has been recommended by the IUIS/WHO study for its specificity and reactivity in a wide range of assay protocols and techniques.

Application

Monoclonal Anti-Human IgG3-FITC antibody produced in mouse has been used in immunohistochemistry and indirect immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Other Notes

In order to obtain best results, it is recommended that each individual user determine working dilutions by titration assay.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Antibody structure, instability, and formulation.
Wang W, et al.
Journal of Pharmaceutical Sciences, 96(1), 1-26 (2007)
IgG Subclasses and Allotypes: From Structure to Effector Functions
Gestur V, et al.
Frontiers in Immunology (2014)
Erdem Tüzün et al.
Acta neuropathologica, 118(6), 737-743 (2009-08-15)
We report the immunopathological analysis of the brain and tumor of two patients who died of anti-NMDAR-associated encephalitis, and of the tumor of nine patients who recovered. Findings included prominent microgliosis and deposits of IgG with rare inflammatory infiltrates in...
Seroprevalence of human herpesvirus 6 in Andino Pune?os (Argentina)
Romagnoli, Pablo Alberto, et al.
Transactions of the Royal Society of Tropical Medicine and Hygiene, 669-672 (2000)
Evidence for antibody-mediated pathogenesis in anti-NMDAR
encephalitis associated with ovarian teratoma
Erdem Tuzun, et al.
Acta Neuropathologica, 118(6) (2009)

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