IP0010

Anti-HA Immunoprecipitation Kit

• NACRES: NA.56

Properties

• antibody product type: primary antibodies
• Quality Level: 200
• clone: monoclonal
• analyte chemical class(es): proteins (hemagglutinin)
• technique(s): immunoprecipitation (IP): suitable
• storage temp.: 2-8°C

Description

General description

Anti-hemagglutinin (HA) Immunoprecipitation Kit is designed to recover maximum amounts of HA-tagged proteins in the immunoprecipitates. The process is carried out in mini-spin columns instead of microcentrifuge tubes to ensure easy washing of antigen-antibody-bound beads with minimal variability.

Application

Anti- hemagglutinin (HA) Immunoprecipitation Kit has been used for immunoprecipitation. It has also been used in in vitro acetylation assay from human embryonic kidney cell lines.

Features and Benefits

• Anti- hemagglutinin (HA) coupled beads enable high specific binding to HA-tagged fusion proteins
• Coupling of Anti-HA antibodies to the beads eliminates other preliminary and calibration steps
• Mini-spin columns enable the minimal loss of anti-HA-bound beads during washing
• The CelLyticTM M cell lysis reagent provides a high yield, rapid and efficient mammalian cell lysis
• Ready-to-use cell lysis reagent and 10X concentrated immunoprecipitation buffer

Kit Components Also Available Separately

• A2095 Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension .5 mL
• C2978 CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 50 mL

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable