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IP0010

Millipore

Anti-HA Immunoprecipitation Kit

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NACRES:
NA.56

antibody product type

primary antibodies

Quality Level

clone

monoclonal

analyte chemical class(es)

proteins (hemagglutinin)

technique(s)

immunoprecipitation (IP): suitable

storage temp.

2-8°C

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Millipore

IP0010

Anti-HA Immunoprecipitation Kit

FLAG® Immunoprecipitation Kit

FLAGIPT1

FLAG® Immunoprecipitation Kit

Anti-HA Magnetic Beads Magnetic agarose, suspension

SAE0197

Anti-HA Magnetic Beads

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

antibody product type

primary antibodies

antibody product type

-

antibody product type

-

antibody product type

-

clone

monoclonal

clone

-

clone

-

clone

-

analyte chemical class(es)

proteins (hemagglutinin)

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

analyte chemical class(es)

-

analyte chemical class(es)

-

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General description

Anti-hemagglutinin (HA) Immunoprecipitation Kit is designed to recover maximum amounts of HA-tagged proteins in the immunoprecipitates. The process is carried out in mini-spin columns instead of microcentrifuge tubes to ensure easy washing of antigen-antibody-bound beads with minimal variability.

Application

Anti- hemagglutinin (HA) Immunoprecipitation Kit has been used for immunoprecipitation. It has also been used in in vitro acetylation assay from human embryonic kidney cell lines.

Features and Benefits

  • Anti- hemagglutinin (HA) coupled beads enable high specific binding to HA-tagged fusion proteins
  • Coupling of Anti-HA antibodies to the beads eliminates other preliminary and calibration steps
  • Mini-spin columns enable the minimal loss of anti-HA-bound beads during washing
  • The CelLyticTM M cell lysis reagent provides a high yield, rapid and efficient mammalian cell lysis
  • Ready-to-use cell lysis reagent and 10X concentrated immunoprecipitation buffer

Kit Components Also Available Separately

Product No.
Description
SDS

  • A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension .5 mLSDS

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 50 mLSDS

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Olga Zurita Rendón et al.
Human molecular genetics, 21(17), 3815-3824 (2012-06-02)
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Krall,P et al.
PLoS ONE, 5(9) (2010)
Lin Jin et al.
PLoS pathogens, 12(5), e1005609-e1005609 (2016-05-19)
Bacterial AvrE-family Type-III effector proteins (T3Es) contribute significantly to the virulence of plant-pathogenic species of Pseudomonas, Pantoea, Ralstonia, Erwinia, Dickeya and Pectobacterium, with hosts ranging from monocots to dicots. However, the mode of action of AvrE-family T3Es remains enigmatic, due
Paola Krall et al.
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Mutations in the TRPC6 calcium channel (Transient receptor potential channel 6) gene have been associated with familiar forms of Focal and Segmental Glomerulosclerosis (FSGS) affecting children and adults. In addition, acquired glomerular diseases are associated with increased expression levels of
Satadru K Lahiri et al.
American journal of cancer research, 6(5), 910-923 (2016-06-14)
Krüppel-like factor 8 (KLF8) plays important roles in cancer and is strictly regulated by various post-translational modifications such as sumoylation, acetylation, ubiquitylation and PARylation. Here we report a novel phosphorylation of KLF8 by ERK2 responsible and critical for the stability

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