MilliporeSigma
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IP0010

Millipore

Anti-HA Immunoprecipitation Kit

NACRES:
NA.56

antibody product type

primary antibodies

Quality Level

clone

monoclonal

analyte chemical class(es)

proteins (hemagglutinin)

technique(s)

immunoprecipitation (IP): suitable

storage temp.

2-8°C

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17-299IP50FLAGIPT1
technique(s)

immunoprecipitation (IP): suitable

technique(s)

western blot: suitable

technique(s)

-

technique(s)

immunoprecipitation (IP): suitable

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

300

antibody product type

primary antibodies

antibody product type

primary antibodies

antibody product type

-

antibody product type

-

clone

monoclonal

clone

-

clone

-

clone

-

analyte chemical class(es)

proteins (hemagglutinin)

analyte chemical class(es)

-

analyte chemical class(es)

-

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

General description

Anti-hemagglutinin (HA) Immunoprecipitation Kit is designed to recover maximum amounts of HA-tagged proteins in the immunoprecipitates. The process is carried out in mini-spin columns instead of microcentrifuge tubes to ensure easy washing of antigen-antibody-bound beads with minimal variability.

Application

Anti- hemagglutinin (HA) Immunoprecipitation Kit has been used for immunoprecipitation. It has also been used in in vitro acetylation assay from human embryonic kidney cell lines.

Features and Benefits

  • Anti- hemagglutinin (HA) coupled beads enable high specific binding to HA-tagged fusion proteins
  • Coupling of Anti-HA antibodies to the beads eliminates other preliminary and calibration steps
  • Mini-spin columns enable the minimal loss of anti-HA-bound beads during washing
  • The CelLyticTM M cell lysis reagent provides a high yield, rapid and efficient mammalian cell lysis
  • Ready-to-use cell lysis reagent and 10X concentrated immunoprecipitation buffer

Kit Components Also Available Separately

Product No.
Description
SDS

  • A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension .5 mL

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 50 mL

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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