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FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

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Synonym(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

usage

 kit sufficient for 50 reactions

Quality Level

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

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This Item
F2426A2220F3290
FLAG® Immunoprecipitation Kit

FLAGIPT1

FLAG® Immunoprecipitation Kit

Millipore

A2220

ANTI-FLAG® M2 Affinity Gel

FLAG® Peptide lyophilized powder

F3290

FLAG® Peptide

usage

 kit sufficient for 50 reactions

usage

-

usage

-

usage

-

Quality Level

300

Quality Level

-

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3xFLAG® tag, DYKDDDDK tag)

analyte chemical class(es)

proteins (FLAG® tag, 3x FLAG®, DYKDDDDK tag)

analyte chemical class(es)

-

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS

  • A2220ANTI-FLAG M2 affinity gel 1 mLSDS

  • SAE01943X FLAG peptide 1 mgSDS

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Mercedes Rodriguez-Teja et al.
Molecular cancer research : MCR, 13(3), 538-547 (2014-11-09)
Epithelial cell-cell contacts maintain normal glandular tissue homeostasis, and their breakage can trigger epithelial-to-mesenchymal transition (EMT), a fundamental step in the development of metastatic cancer. Despite the ability of C-type lectin domains (CTLD) to modulate cell-cell adhesion, it is not
Kuancan Liu et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(3), 901-913 (2020-01-29)
Esophageal squamous cell carcinoma (ESCC) is a predominant cancer type in developing countries such as China, where ESCC accounts for approximately 90% of esophageal malignancies. Lacking effective and targeted therapy contributes to the poor 5-year survival rate. Recent studies showed
Kalpana Kannan et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(11), E1272-E1277 (2015-03-04)
High-grade serous ovarian cancer (HGSC) is among the most lethal forms of cancer in women. Excessive genomic rearrangements, which are expected to create fusion oncogenes, are the hallmark of this cancer. Here we report a cancer-specific gene fusion between BCAM
Konstantinos Koudounas et al.
Plant physiology, 174(3), 1371-1383 (2017-05-10)
Oleuropein, a terpene-derived glycosylated secoiridoid biosynthesized exclusively by members of the Oleaceae family, is involved in a two-component defense system comprising a β-glucosidase that activates oleuropein into a toxic glutaraldehyde-like structure. Oleuropein and its deglycosylated derivatives have high pharmaceutical interest.
Andrew S Terker et al.
American journal of physiology. Renal physiology, 315(4), F781-F790 (2018-02-08)
With no lysine kinase 4 (WNK4) is essential to activate the thiazide-sensitive NaCl cotransporter (NCC) along the distal convoluted tubule, an effect central to the phenotype of familial hyperkalemic hypertension. Although effects on potassium and sodium channels along the connecting

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