FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

Synonym(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

Quality Level

usage

 kit sufficient for 50 reactions

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

application(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • A2220ANTI-FLAG M2 affinity gel 1 mL

  • SAE01943X FLAG peptide 1 mg

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Certificate of Analysis
Certificate of Origin
With no lysine kinase 4 modulates sodium potassium 2 chloride cotransporter activity in vivo.
Terker, et al.
American Journal of Physiology: Renal Physiology, 315, F781-F790 (2019)
null
null
Mateusz Wydro et al.
Nucleic acids research, 38(11), 3732-3742 (2010-02-11)
Mammalian mitochondria contain their own genome that is almost fully transcribed from both strands, generating polycistronic RNA units that are processed and matured. The mitochondrial mRNA is modified by oligo- or polyadenylation at the 3' termini, but the exact function...
The C-Domain of Oleuropein ?-Glucosidase Assists in Protein Folding and Sequesters the Enzyme in Nucleus.
Koudounas, et al.
Plant Physiology, 174, 1371-1383 (2018)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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