FLAGIPT1

FLAG^® Immunoprecipitation Kit

• Synonym(s): Anti-ddddk, Anti-dykddddk
• NACRES: NA.32

Properties

• usage: 1 kit sufficient for 50 reactions
• Quality Level: 300
• analyte chemical class(es): proteins (FLAG® Affinity Gels)
• technique(s): immunoprecipitation (IP): suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Browse additional application references in our FLAG^® Literature portal.

Features and Benefits

• Utilizes highly specific ANTI-FLAG M2 affinity gel
• No preliminary steps or calibrations
• 3X FLAG^™ peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• A2220 ANTI-FLAG M2 affinity gel 1 mL
• SAE0194 3X FLAG peptide 1 mg

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319 - H412
• Precautionary Statements: P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3