MilliporeSigma
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M7905

Sigma-Aldrich

Monoclonal Anti-Myosin Light Chain Kinase antibody produced in mouse

clone K36, ascites fluid

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Synonym(s):
Anti-KRP, Anti-MLCK, Anti-MLCK1, Anti-MLCK108, Anti-MLCK210, Anti-MMIHS, Anti-MMIHS1, Anti-MSTP083, Anti-MYLK1, Anti-smMLCK
MDL number:
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

K36, monoclonal

contains

15 mM sodium azide

species reactivity

avian, mammals

technique(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:10,000

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYLK(4638)

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This Item
M4401M6068M7786
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

ascites fluid

clone

K36, monoclonal

clone

MY-21, monoclonal

clone

polyclonal

clone

hSM-V, monoclonal

species reactivity

avian, mammals

species reactivity

human, chicken, pig, bovine, rabbit

species reactivity

human (predicted), chicken

species reactivity

guinea pig, human, pig, canine, rabbit, chicken

technique(s)

immunoprecipitation (IP): suitable, microarray: suitable, western blot: 1:10,000

technique(s)

indirect immunofluorescence: 1:200 using human or chicken fibroblasts, western blot: suitable using denatured and reduced myosin of muscle

technique(s)

western blot: 1:1,000 using chicken gizzard myosin II phosphorylated in vitro by PAK

technique(s)

immunohistochemistry: 1:500 using and animal frozen section using acetone fixed human., immunohistochemistry: suitable using using methacarn-fixed, paraffin-embedded sections of human and animal tissue, immunoprecipitation (IP): suitable, western blot: suitable

General description

Monoclonal Anti-Myosin Light Chain Kinase (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin light chain kinase (MLCK) is a calcium/calmodulin-dependent enzyme (approx.160 kD). It is expressed in skeletal, cardiac, smooth and mammalian non-muscle cells. MLCK is encoded by the MYLK1 gene. This gene encodes telokin, non-muscle and the smooth muscle isoforms of MLCK. MLCK gene is mapped to the human chromosome location 3q21.1.

Specificity

In immunoblotting, the antibody recognizes the myosin light chain kinase of smooth muscle from various sources including chicken gizzard, turkey gizzard, and pig stomach as well as the myosin light chain kinase of non-muscle cells such as cultured fibroblasts.

Immunogen

chicken gizzard myosin light chain kinase.

Application

Monoclonal anti-myosin light chain kinase antibody has been used in:
  • immunohistochemistry
  • immunoblot (diluted 1:10,000) analysis
  • flow cytometry

Biochem/physiol Actions

Myosin Light Chain Kinase (MLCK) has a pivotal role in phosphorylating myosin regulatory light chains and hence facilitates its interaction with actin filaments for smooth muscle contraction. Monoclonal anti-myosin light chain kinase antibody can be used for studying the function of the enzyme and its interactions with other cell components. It can also be used in microarray and immunoprecipitation. Mouse anti-myosin light chain kinase antibody reacts specifically with myosin light chain kinase present in smooth muscle of chicken gizzard, turkey gizzard, and pig stomach. This product also shows reactivity for myosin light chain kinase of non-muscle cells like cultured fibroblasts.
The catalytic subunit of cardiac and skeletal muscle myosin light chain kinase is in the range of 80-95 kDa. Proteolysis of the myosin light chain kinase from chicken gizzard produces a 64 kDa fragment that neither binds to Ca2+/calmodulin nor exhibits catalytic activity and a 61 kDa peptide that is active in the absence of Ca2+/calmodulin.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ze-Yan Yu et al.
PloS one, 9(6), e99024-e99024 (2014-06-12)
To determine the mechanisms by which the α1A-adrenergic receptor (AR) regulates cardiac contractility. We reported previously that transgenic mice with cardiac-restricted α1A-AR overexpression (α1A-TG) exhibit enhanced contractility but not hypertrophy, despite evidence implicating this Gαq/11-coupled receptor in hypertrophy. Contractility, calcium
Null mutation in macrophage migration inhibitory factor prevents muscle cell loss and fibrosis in partial bladder outlet obstruction.
John A. Taylor
American Journal of Physiology: Renal Physiology, 291(6), F1343-F1353 (2006)
Elena Kassianidou et al.
Molecular biology of the cell, 28(26), 3832-3843 (2017-10-20)
The assembly and mechanics of actomyosin stress fibers (SFs) depend on myosin regulatory light chain (RLC) phosphorylation, which is driven by myosin light chain kinase (MLCK) and Rho-associated kinase (ROCK). Although previous work suggests that MLCK and ROCK control distinct
Daniel A Emmert et al.
American journal of physiology. Cell physiology, 286(1), C8-21 (2003-09-12)
Thus far, determining the relative contribution of Ca2+/calmodulin-dependent myosin light chain kinase (MLCK) and Ca2+-independent Rho-kinase pathways to myosin II activation and contraction has been difficult. In this study, we characterize the role of Rho-kinase in a rat embryo fibroblast
T Jindo et al.
Toxicologic pathology, 29(6), 607-616 (2002-01-17)
Methoxyacetic acid (MAA) is a major metabolite of ethylene glycol monomethyl ether (EGME). Previous investigations of the testicular lesion induced by EGME have found that dividing meiotic cells are the most sensitive, although several stages of spermatocytes are also vulnerable.

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