This product is the hydrate form of methotrexate. The maximum allowable water content is 12% and is reported on the lot-specific Certificate of Analysis. Dessicated storage is not necessary but is up to the end user's preference to do so. Any resulting moisture loss should be considered when preparing stock solutions. Please see the link below to review the product datasheet for A6770. This product is not tested for cell culture use, but the information contained in the document would apply to M8407.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/249/853/a6770pis.pdf
M8407
Methotrexate hydrate
powder, suitable for cell culture, BioReagent
Synonym(s):
4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate
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About This Item
Empirical Formula (Hill Notation):
C20H22N8O5 · xH2O
CAS Number:
Molecular Weight:
454.44 (anhydrous basis)
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75
MDL number:
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Product Name
Methotrexate hydrate, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)
SMILES string
[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
InChI
1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
InChI key
FPJYMUQSRFJSEW-ZOWNYOTGSA-N
product line
BioReagent
assay
≥98% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: insoluble
antibiotic activity spectrum
neoplastics
parasites
mode of action
enzyme | inhibits
storage temp.
−20°C
Quality Level
Gene Information
human ... DHFR(1719)
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Related Categories
1 of 4
This Item | A6770 | M9929 | D9571 |
|---|---|---|---|
| antibiotic activity spectrum neoplastics, parasites | antibiotic activity spectrum - | antibiotic activity spectrum parasites | antibiotic activity spectrum - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) activity assay: suitable | technique(s) - |
| mode of action enzyme | inhibits | mode of action - | mode of action DNA synthesis | mode of action - |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 100 |
| product line BioReagent | product line - | product line - | product line - |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay 98.0-102.0% | assay ≥98% (HPLC) |
Application
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Methotrexate hydrate has been used:
- as a dihydrofolate (DHFR) inhibitor in sphere forming assay culture[1]
- as a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells[2][3]
- in viability and co-culture assays [4]
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems.
Biochem/physiol Actions
Methotrexate is a folic acid antagonist.[5]
Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.
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Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Folate supplementation during methotrexate therapy for patients with psoriasis
Strober BE and Menon K
Journal of the American Academy of Dermatology, 53(4), 652-659 (2005)
Methylthioadenosine (MTA) Rescues Methylthioadenosine Phosphorylase (MTAP)-deficient tumors from purine synthesis inhibition in vivo via non-autonomous adenine supply
Ruefli-Brasse A, et al.
Journal of Cancer Therapy, 2(4), 523-523 (2011)
Localizations of Na+-d-glucose cotransporters SGLT1 and SGLT2 in human kidney and of SGLT1 in human small intestine, liver, lung, and heart
Vrhovac I, et al.
Pflugers Archiv : European Journal of Physiology, 467(9), 1881-1898 (2015)
Comparative metabolic analysis of CHO cell clones obtained through cell engineering, for IgG productivity, growth and cell longevity
Wilkens CA and Gerdtzen ZP
Testing, 10(3), e0119053-e0119053 (2015)
Yunqian Qiao et al.
Nature communications, 12(1), 6360-6360 (2021-11-06)
Expression of the cell surface receptor CD137 has been shown to enhance anti-cancer T cell function via engagement with its natural ligand 4-1BBL. CD137 ligation with engineered ligands has emerged as a cancer immunotherapy strategy, yet clinical development of agonists
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Does the powder form of this product M8407 (methotrexate hydrate) need to be stored in a desiccator?
1 answer-
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Regarding to the answer for making a solution of Methotrexate hydrate, can we use Sodium Bicarbonate to dissolve Methotrexate hydrate for cell culture?
1 answer-
This product is not directly soluble in water and is tested for solubility in 1M Ammonium hydroxide at 50 mg/mL. This material is also soluble in 1M Sodium hydroxide. Alternatively, anhydrous DMSO may also be used to dissolve this material at 90 mg/ml. An identical Methotrexate hydrate product, A6770, has a datasheet posted to the website. This datasheet included additional information regarding general use and solution stability. Please see the link below to review this document:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/249/853/a6770pis.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I make a solution of Methotrexate hydrate, Product M8407?
1 answer-
Methoxtrexate is soluble in 1M NaOH or NH4OH (10 mg/ml). For use in cell culture, sodium carbonate buffer (pH 9.0-9.5) can be used to dissolve methotrexate.
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How often does Methotrexate hydrate, Product M8407, need to be added to the media during selection?
1 answer-
Fresh methotrexate should be added each time the culture media is changed. Regardless of cell growth, most media will need to be changed at least once a week.
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How do I store a stock solution of Methotrexate hydrate, Product M8407?
1 answer-
A sterile solution of methotrexate can be stored at 2-8 °C for approximately 30 days.
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How do I sterilize a solution of Methotrexate hydrate, Product M8407?
1 answer-
A solution of methotrexate can be sterile filtered using a 0.22 mm filter
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