The specific composition of the elution buffer E7777 is considered proprietary. However it is a Tris-HCL solution without EDTA.
NA1020
GenElute™ PCR Clean-Up Kit
sufficient for 70 purifications
GenElute™ PCR Clean-Up Kit
Synonym(s):
Gen Elute, PCR Purification
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$197.00
$197.00
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.52
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usage
sufficient for 70 purifications
technique(s)
DNA purification: suitable
storage temp.
15-25°C
1 of 4
This Item | NA1111 | NA9604 | G1N10 |
|---|---|---|---|
| usage sufficient for 70 purifications | usage sufficient for 70 purifications | usage sufficient for 4 96-well plate purifications | usage sufficient for 10 purifications |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) - |
PCR9601 | - | NA0150, NA0150S, NA0160 | G1N350, G1N70, G1N9602, G1N9604 |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Application
GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.[1][2][3]
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Biochem/physiol Actions
The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).
Features and Benefits
- Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Removes over 99% of primers and other components
- Eliminates the need to remove mineral oil by organic extraction
- 40% more purification preps supplied than market leader
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Purifies up to 100 μL or 10 μg of PCR amplified DNA in 8 minutes
- Removes over 99% of primers and other components
Other Notes
The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the elution buffer made of? As it can be replaced with water, is it just water that is provided?
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Does this kit remove proteins from PCR product?
1 answer-
This is a post-pcr clean up kit. The single or double-stranded amplification products are bound to the silica membrane. Then all non-DNA material such as primers, nucleotides, and polymerase are washed away. Finally the purified product is eluted. Anything that isn't bound will be removed.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product NA1020, GenElute™ PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?
1 answer-
Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
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Can Product No. NA1020, GenElute™ PCR Clean-Up Kit, be used to clean up PCR products larger than 10 kb?
1 answer-
We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well. That said, the bound DNA might be more difficult to elute. Heating the elution solution to 60-65°C may help with elution. Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
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When using Product No. NA1020, GenElute™ PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?
1 answer-
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
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When using GenElute™ PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?
1 answer-
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
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Can GenElute™ PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?
1 answer-
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
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