Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Central nervous system
8A - Combustible, corrosive hazardous materials
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Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well. That said, the bound DNA might be more difficult to elute. Heating the elution solution to 60-65°C may help with elution. Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
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