P0060

PLK1, active, His tagged human

Name: PLK1, active, His tagged human
Brand: SIGMA

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

STPK13

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

Key Documents

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Product Name

PLK1, active, His tagged human, PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

14-20 nmol/min·mg

mol wt

~70 kDa

UniProt accession no.

P53350

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

human ... PLK1(5347)

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This Item	SRP0360	K3393	SRP5246
Sigma-Aldrich P0060 PLK1, active, His tagged human	Sigma-Aldrich SRP0360 PLK1 Polo Box Domain active human	Sigma-Aldrich K3393 PDK1, active, His tagged human	Sigma-Aldrich SRP5246 PAK1, active, GST tagged human
assay ≥70% (SDS-PAGE)	assay ≥90% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
Gene Information human ... PLK1(5347)	Gene Information human ... PLK1(5347)	Gene Information human ... PDPK1(5170)	Gene Information human ... PAK1(5058)
specific activity 14-20 nmol/min·mg	specific activity -	specific activity 102-138 nmol/min·mg	specific activity 63-85 nmol/min·mg
form buffered aqueous glycerol solution	form aqueous solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in E. coli	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
UniProt accession no. P53350	UniProt accession no. P53350	UniProt accession no. Q6A1A2, O15530	UniProt accession no. -

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Biochem/physiol Actions

PLK1 (polo like kinase 1) is predominantly involved in mitosis. It mediates the phosphorylation of cyclin B1/CDK1 (cyclin dependent kinase 1) complex, and activation of the anaphase promoting complex. PLK1 has anti-apoptotic function. Upregulation of gene is observed in different types of cancer, such as colorectal cancer and is regarded as an oncogene. PLK1 is involved in checkpoint recovery and adaptation following DNA damage.[1][2][3]

General description

PLK1 (polo like kinase 1) gene is mapped to human chromosome 16p12.2. It codes for the protein serine/threonine kinase.[1][4]

Physical form

Supplied in 50 mM Sodium Phosphate, pH 7.0, with 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H360D

pcodes

P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Polo-like kinase 1 expression is suppressed by CCAAT/enhancer-binding protein a to mediate colon carcinoma cell differentiation and apoptosis.

Dasgupta N

Biochimica et Biophysica Acta (2017)

PLK1 inhibition enhances temozolomide efficacy in IDH1 mutant gliomas.

Koncar RF

Oncotarget (2017)

Polo-like Kinase 1 as a potential therapeutic target in Diffuse Intrinsic Pontine Glioma.

Amani V

BMC Cancer (2016)

Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation.

E A Nigg et al.

Experimental cell research, 229(2), 174-180 (1996-12-15)

During mitosis, the vertebrate cell nucleus undergoes profound changes in architecture. At the onset of mitosis, the nuclear envelope breaks down, the nuclear lamina is depolymerized, and interphase chromatin is condensed to chromosomes. Concomitantly, cytoplasmic microtubules are reorganized into a

Expression of the microtubule-associated protein MAP9/ASAP and its partners AURKA and PLK1 in colorectal and breast cancers.

Rouquier S

Disease Markers (2014)

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