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MilliporeSigma

S6147

Sephadex® G-100

DNA grade, BioReagent, Molecular Biology, medium

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25 G

$371.00

100 G

$1,011.50

$371.00

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About This Item

CAS Number:
9050-94-6
UNSPSC Code:
23151817
NACRES:
NA.52
MDL number:
MFCD00002744

Key Documents

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Product Name

Sephadex® G-100, DNA grade, BioReagent, Molecular Biology, medium

grade

Molecular Biology

product line

BioReagent

form

beads

technique(s)

buffer exchange: suitable

matrix active group

phase

swelling

1 g swells to 9-11 mL

bead diameter

40-120 μm

pore size

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

application(s)

life science and biopharma

compatibility

Cytiva

foreign activity

DNase, RNase, none detected

storage temp.

room temp

Quality Level

200

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This Item
GE17-0060-01G10050G100120
Sephadex® G-100 DNA grade, BioReagent, Molecular Biology, medium

Sigma-Aldrich

S6147

Sephadex® G-100

Sephadex® G-100 Cytiva 17-0060-01, pack of 100 g

GE17-0060-01

Sephadex® G-100

Sephadex® G-100 Superfine

Sigma-Aldrich

G10050

Sephadex® G-100

Sephadex® G-100 Medium

Sigma-Aldrich

G100120

Sephadex® G-100

application(s)

life science and biopharma

application(s)

-

application(s)

life science and biopharma

application(s)

-

compatibility

Cytiva

compatibility

-

compatibility

Cytiva

compatibility

-

technique(s)

buffer exchange: suitable

technique(s)

-

technique(s)

affinity chromatography: suitable

technique(s)

LPLC: suitable

matrix active group

phase

matrix active group

-

matrix active group

phase

matrix active group

polymer

grade

DNA grade

grade

-

grade

-

grade

-

product line

BioReagent

product line

-

product line

-

product line

-

Application

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.[1]

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

General description

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000510327
0000510327
0000483387
0000483387
0000467894

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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
Sanjiv K Mishra et al.
Protein expression and purification, 80(2), 234-238 (2011-07-13)
C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100 mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel
Fernanda O Lemos et al.
European journal of dermatology : EJD, 21(5), 722-730 (2011-07-09)
Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of
Qiang Luo et al.
Journal of medicinal food, 14(12), 1609-1616 (2011-09-01)
Crude water-soluble Chinese truffle Tuber indicum polysaccharide (TIP) was extracted from the fruiting bodies with water and then successively purified by DEAE-cellulose 52 and Sephadex G-100 column chromatography, yielding two major polysaccharide fractions: TIP1-1 and TIP2-1. High-performance gel permeation chromatography
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