SRP0115

Sirtuin 1 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

• Synonym(s): SIR2, SIRT1, Sir2A, sir2-like 1
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥60% (SDS-PAGE)
• form: aqueous solution
• mol wt: 87.2 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• NCBI accession no.: NM_012238
• UniProt accession no.: Q96EB6
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SIRT1(23411)

Description

General description

Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed in E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable