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SRP0115

Sigma-Aldrich

Sirtuin 1 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

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Synonym(s):
SIR2, SIRT1, Sir2A, sir2-like 1
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥60% (SDS-PAGE)

form

aqueous solution

mol wt

87.2 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SIRT1(23411)

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vibrant-m

SRP0115

Sirtuin 1 human

vibrant-m

SRP0116

Sirtuin 2 human

vibrant-m

SRP0120

Sirtuin 6 human

vibrant-m

SRP0117

Sirtuin 3 human

biological source

human

biological source

human

biological source

human

biological source

human

assay

≥60% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

packaging

pkg of 100 μg

packaging

pkg of 100 μg

packaging

pkg of 100 μg

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed in E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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SIRT 1 Overexpression is Associated with Metastasis of Pancreatic Ductal Adenocarcinoma (PDAC) and Promotes Migration and Growth of PDAC Cells.
Li S, et al.
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 1593-1600 (2016)
[SIRT1].
Hattori Y and Ihara M.
Nihon Rinsho. Japanese Journal of Clinical Medicine, 74(4), 589-594 (2016)
Wen-Li Mu et al.
Stem cells (Dayton, Ohio), 33(7), 2135-2147 (2015-05-06)
Mouse somatic cells can be reprogrammed into induced pluripotent stem cells by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. Together with Oct4, Sox2 plays a major role as a master endogenous pluripotent genes trigger in
Yanxiang Li et al.
The Journal of nutritional biochemistry, 26(11), 1338-1347 (2015-08-25)
Nicotinic acid (NA) has recently been shown to inhibit inflammatory response in cardiovascular disease. Sirtuin1 (SIRT1), a NAD(+)-dependent class III histone deacetylase, participates in the regulation of cellular inflammation. We hypothesized that dietary supplementation of NA could attenuate vascular inflammation
Yun H Kim et al.
Vascular pharmacology, 72, 35-42 (2015-05-15)
Silent mating type information regulation 2 homolog 1 (SIRT1) is known as a key regulator in the protection of various vascular disorders, however, no direct evidences have been reported in the progression of atherosclerosis. Considering the pivotal role of matrix

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