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SRP5270

Sigma-Aldrich

SIRT1, active, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
SIR2L1
CAS Number:
1391438-73-5
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

1573-2415 RLU/min·ng

mol wt

~84 kDa

NCBI accession no.

NM_012238

application(s)

genomic analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SIRT1(23411)

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SIRT1, active, His tagged human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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SRP5274

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SIRT6, active, His tagged human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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SIRT6, active, His tagged human

SIRT3, active, GST tagged human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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SRP5275

SIRT3, active, GST tagged human

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

specific activity

1573-2415 RLU/min·ng

specific activity

112-139 RLU/min·ng

specific activity

96-144 RLU/min·ng

specific activity

123-196 RLU/min·ng

mol wt

~84 kDa

mol wt

~46 kDa

mol wt

~39 kDa

mol wt

~66 kDa

General description

Sirtuin 1 (SIRT1) is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. SIRT1 is also called as NAD-dependent deacetylase sirtuin-1. This gene is located on human chromosome 10q21.3.

Biochem/physiol Actions

Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells. It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition. SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level. It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Influence of SIRT1 polymorphisms for diabetic foot susceptibility and severity
Peng Y, et al.
Medicine, 97(28) (2018)
SIRT1 gene polymorphisms associated with decreased risk of atherosclerotic coronary artery disease
Nasiri M, et al.
Gene (2018)

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