T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

Name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride
Brand: SIGMA

chromogenic, tablet

Synonym(s):

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₂₀N₂ · 2HCl

CAS Number:

64285-73-0

Molecular Weight:

313.27

NACRES:

NA.83

PubChem Substance ID:

24900140

UNSPSC Code:

12352204

EC Number:

264-769-6

MDL number:

MFCD00012961

Key Documents

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Product Name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

SMILES string

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

form

tablet

mp

≥300 °C

storage temp.

2-8°C

Quality Level

200

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Show Differences

1 of 4

This Item	693014	96738	SML3593
Sigma-Aldrich T3405 3,3′,5,5′-Tetramethylbenzidine dihydrochloride	Sigma-Aldrich 693014 (S)-H₈-BINAP	Sigma-Aldrich 96738 (R)-(+)-5,5′-Dichloro-2,2′-bis(diphenylphosphino)-6,6′-dimethoxy-1,1′-biphenyl	Sigma-Aldrich SML3593 Hexachlorophene
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
form tablet	form powder	form crystals	form powder
storage temp. 2-8°C	storage temp. -	storage temp. -	storage temp. 2-8°C
mp ≥300 °C	mp -	mp -	mp -

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the

anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera[1]
anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate[2]
in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients[3]

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000427553

0000408058

0000379104

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Identification of a protective B-cell epitope of the Staphylococcus aureus GapC protein by screening a phage-displayed random peptide library.

Mengyao Wang et al.

PloS one, 13(1), e0190452-e0190452 (2018-01-06)

The impact of epidemic Staphylococcus aureus (S. aureus) on public health is increasing. Because of the abuse of antibiotics, the antibiotic resistance of S. aureus is increasing. Thus, there is an urgent need to develop new immunotherapies and immunoprophylaxes. Previous

Ultrasensitive colorimetric determination of silver(I) based on the peroxidase mimicking activity of a hybrid material composed of graphitic carbon nitride and platinum nanoparticles.

Shurong Tang et al.

Mikrochimica acta, 185(5), 273-273 (2018-05-01)

A hybrid material composed of graphitic carbon nitride (g-C3N4) and platinum nanoparticles (PtNPs) with peroxidase mimicking activity was used to design a rapid, sensitive and low-cost colorimetric method for the determination of Ag(I). The g-C3N4-PtNPs hybrid was synthesized by reduction

Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection.

Sandra Oloketuyi et al.

Biosensors, 11(12) (2021-12-24)

C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher

CD45 isoform expression is associated with different susceptibilities of human naive and effector CD4+ T cells to respond to IL-4

Stutz A, et al.

European Journal of Immunology, 35(2), 575-583 (2005)

Nonhuman primate infants have an impaired respiratory but not systemic IgG antibody response following influenza virus infection.

Beth C Holbrook et al.

Virology, 476, 124-133 (2014-12-30)

Respiratory infection of young infants results in increased morbidity and mortality compared to infection of adults. In spite of the significance of this health issue, our understanding of the immune response elicited in infants especially in the respiratory tract is

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