• Home
  • Search Results
  • Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche.

Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche.

Immunity (2019-09-29)
Johnny Bonnardel, Wouter T'Jonck, Djoere Gaublomme, Robin Browaeys, Charlotte L Scott, Liesbet Martens, Bavo Vanneste, Sofie De Prijck, Sergei A Nedospasov, Anna Kremer, Evelien Van Hamme, Peter Borghgraef, Wendy Toussaint, Pieter De Bleser, Inge Mannaerts, Alain Beschin, Leo A van Grunsven, Bart N Lambrecht, Tom Taghon, Saskia Lippens, Dirk Elewaut, Yvan Saeys, Martin Guilliams

Macrophages are strongly adapted to their tissue of residence. Yet, little is known about the cell-cell interactions that imprint the tissue-specific identities of macrophages in their respective niches. Using conditional depletion of liver Kupffer cells, we traced the developmental stages of monocytes differentiating into Kupffer cells and mapped the cellular interactions imprinting the Kupffer cell identity. Kupffer cell loss induced tumor necrosis factor (TNF)- and interleukin-1 (IL-1) receptor-dependent activation of stellate cells and endothelial cells, resulting in the transient production of chemokines and adhesion molecules orchestrating monocyte engraftment. Engrafted circulating monocytes transmigrated into the perisinusoidal space and acquired the liver-associated transcription factors inhibitor of DNA 3 (ID3) and liver X receptor-α (LXR-α). Coordinated interactions with hepatocytes induced ID3 expression, whereas endothelial cells and stellate cells induced LXR-α via a synergistic NOTCH-BMP pathway. This study shows that the Kupffer cell niche is composed of stellate cells, hepatocytes, and endothelial cells that together imprint the liver-specific macrophage identity.

Product Number
Product Description

HEPES, ≥99.5% (titration)
Goat serum
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Bovine Serum Albumin, fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)
Sodium chloride, anhydrous, Redi-Dri, free-flowing, ACS reagent, ≥99%
Diphtheria Toxin from Corynebacterium diphtheriae, lyophilized powder, Product is in unnicked form
Phenol Red, powder, BioReagent, suitable for cell culture
Calcium chloride, AnhydroBeads, −10 mesh, ≥99.9% trace metals basis
Sodium bicarbonate, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.7%
Rat serum
L-Aspartic acid, BioXtra, ≥99% (HPLC)
Dipeptidyl Peptidase III human, recombinant, expressed in Sf9 cells

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.