MilliporeSigma
  • Home
  • Search Results
  • Influence of Pluronic 85 and ketoconazole on disposition and efficacy of ivermectin in sheep infected with a multiple resistant Haemonchus contortus isolate.

Influence of Pluronic 85 and ketoconazole on disposition and efficacy of ivermectin in sheep infected with a multiple resistant Haemonchus contortus isolate.

Veterinary parasitology (2012-03-21)
D J Bartley, A A Morrison, J Dupuy, Y Bartley, J F Sutra, C Menez, M Alvinerie, F Jackson, L Devin, A Lespine
ABSTRACT

Non-specific mechanisms involving ATP-binding cassette drug efflux transporters may play an important role in xenobiotic clearance in ovine gastro-intestinal nematodes. By using transporter inhibitors, the aim of this trial was to assess the possibility of increasing drug bioavailability in the host in an attempt to improve treatment efficacy. Thirty-six lambs were infected with 5000 multiple-drug resistant Haemonchus contortus third stage larvae and separated into six groups (n=6): ivermectin alone (IVM; 0.2 mg/kg body-weight, BW), ketoconazole alone (KET; 10 mg/kg BW), Pluronic 85 alone (P85; 4 mg/kg BW), IVM+KET, IVM+P85 or untreated control. Ivermectin was administered once on day 28 post-infection for all appropriate groups, whereas KET and P85 were administered as five separate doses on day 26-30 post-infection inclusive. The resultant data showed that concomitant administration of KET or P85 with IVM induced increases in plasma and tissue concentrations of IVM in treated animals, resulting in a two-fold increase in the area under the time-concentration curve (p<0.05). Faecal egg counts and worm burdens of the IVM+KET and IVM+P85 groups were lower than in the untreated, KET and P85 alone control animals. Worm burdens were reduced by between 16% and 51% with IVM+KET and IVM+P85 respectively compared to untreated control animals. The co-administration of P85 with IVM increased the efficacy by 34%, compared with IVM alone, in terms of worm count reduction of the multi-resistant isolate of H. contortus.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poloxamer 407, purified, non-ionic
Sigma-Aldrich
Kolliphor® P 188, solid
Sigma-Aldrich
Synperonic® PE/P84
Sigma-Aldrich
Kolliphor® P 188
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,800
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,100
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~2,700
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,000
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,900
Sigma-Aldrich
Synperonic® PE P105, surfactant
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~4,400
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~14,600
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~5,800
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~3,300
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~8,400
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,900
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~2,000
Sigma-Aldrich
Synperonic® F 108, surfactant, non-ionic
Sigma-Aldrich
Pluronic® F-127, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Poloxamer 188 solution, 10%, sterile-filtered, BioReagent, suitable for insect cell culture
Sigma-Aldrich
Kolliphor® P 407, oxyethylene 71.5-74.9 %
Sigma-Aldrich
Kolliphor® P 407 micro
Sigma-Aldrich
Kolliphor® P 188 micro