A novel reusable PAni-PVA-Amylase film: activity and analysis.

Colloids and surfaces. B, Biointerfaces (2013-02-26)
Susmita Singh, Jyoti Prasad Saikia, Alak Kumar Buragohain
ABSTRACT

Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25°C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe(2+) inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
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Sigma-Aldrich
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Sigma-Aldrich
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Sigma-Aldrich
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Sigma-Aldrich
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Sigma-Aldrich
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Sigma-Aldrich
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Sigma-Aldrich
α-Amylase from human saliva, Type XIII-A, lyophilized powder, 300-1,500 units/mg protein
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Poly(vinyl alcohol), Mw 13,000-23,000, 87-89% hydrolyzed
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Poly(vinyl alcohol), Mw 146,000-186,000, 99+% hydrolyzed
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Poly(vinyl alcohol), Mw 85,000-124,000, 99+% hydrolyzed
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Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Poly(vinyl alcohol), Mw 31,000-50,000, 98-99% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), average Mw 13,000-23,000, 98% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), average Mw 85,000-124,000, 87-89% hydrolyzed
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Sigma-Aldrich
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Sigma-Aldrich
Poly(vinyl alcohol), average Mw 31,000-50,000, 87-89% hydrolyzed
Sigma-Aldrich
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Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
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Sigma-Aldrich
Poly(vinyl alcohol), average Mw 146,000-186,000, 87-89% hydrolyzed
Sigma-Aldrich
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Sigma-Aldrich
Mowiol® 4-98, Mw ~27,000
Sigma-Aldrich
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