• Home
  • Search Results
  • TET1 controls CNS 5-methylcytosine hydroxylation, active DNA demethylation, gene transcription, and memory formation.

TET1 controls CNS 5-methylcytosine hydroxylation, active DNA demethylation, gene transcription, and memory formation.

Neuron (2013-09-21)
Garrett A Kaas, Chun Zhong, Dawn E Eason, Daniel L Ross, Raj V Vachhani, Guo-Li Ming, Jennifer R King, Hongjun Song, J David Sweatt
ABSTRACT

Dynamic changes in 5-methylcytosine (5mC) have been implicated in the regulation of gene expression critical for consolidation of memory. However, little is known about how these changes in 5mC are regulated in the adult brain. The enzyme methylcytosine dioxygenase TET1 (TET1) has been shown to promote active DNA demethylation in the nervous system. Therefore, we took a viral-mediated approach to overexpress the protein in the hippocampus and examine its potential involvement in memory formation. We found that Tet1 is a neuronal activity-regulated gene and that its overexpression leads to global changes in modified cytosine levels. Furthermore, expression of TET1 or a catalytically inactive mutant (TET1m) resulted in the upregulation of several neuronal memory-associated genes and impaired contextual fear memory. In summary, we show that neuronal Tet1 regulates DNA methylation levels and that its expression, independent of its catalytic activity, regulates the expression of CNS activity-dependent genes and memory formation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium chloride, ACS reagent, 99.0-100.5%
Sigma-Aldrich
Potassium chloride, for molecular biology, ≥99.0%
Sigma-Aldrich
Potassium chloride, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%
Supelco
Potassium chloride solution, for Ag/AgCl electrodes, ~3 M KCl, saturated with silver chloride
Sigma-Aldrich
Potassium chloride, BioXtra, ≥99.0%
Sigma-Aldrich
Potassium chloride solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Potassium chloride, ReagentPlus®, ≥99.0%
Supelco
Potassium chloride solution, BioUltra, ~3 M in H2O
Sigma-Aldrich
Potassium chloride, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E508, 99-100.5% (AT), ≤0.0001% Al
Sigma-Aldrich
Potassium chloride solution, 0.075 M, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
Cytosine, ≥99%
Sigma-Aldrich
Bis(2,2,2-trifluoroethyl) ether, 98%
Sigma-Aldrich
Potassium chloride, 99.999% trace metals basis
Sigma-Aldrich
Potassium chloride, puriss. p.a., ≥99.5% (AT)
Supelco
ISA (ionic strength adjustment solution: 1 M KCl), 1 M KCl
Sigma-Aldrich
Potassium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Supelco
Potassium chloride solution, conductance standard B acc. to ISO 7888, 0.01 M KCl
Supelco
Potassium chloride solution, conductance standard A acc. to ISO 7888, 0.1 M KCl
Supelco
Potassium chloride solution, conductance standard C acc. to ISO 7888, 0.001 M KCl
Sigma-Aldrich
Potassium chloride, ≥99.99% trace metals basis
Sigma-Aldrich
Potassium chloride, tested according to Ph. Eur.
Sigma-Aldrich
Potassium chloride, AnhydroBeads, −10 mesh, 99.99% trace metals basis
5-Methylchrysene, BCR®, certified reference material
Sigma-Aldrich
Potassium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis