MilliporeSigma
  • Identification, quantification, and sensory characterization of steviol glycosides from differently processed Stevia rebaudiana commercial extracts.

Identification, quantification, and sensory characterization of steviol glycosides from differently processed Stevia rebaudiana commercial extracts.

Journal of agricultural and food chemistry (2014-11-14)
María Inés Espinoza, Jean-Paul Vincken, Mark Sanders, Cristian Castro, Markus Stieger, Eduardo Agosin
ABSTRACT

Stevia rebaudiana is known for its sweet-tasting ent-kaurene diterpenoid glycosides. Several manufacturing strategies are currently employed to obtain Stevia sweeteners with the lowest possible off-flavors. The chemical composition of four commercial S. rebaudiana extracts, obtained by different technologies, was characterized using UHPLC-ESI-MS(n). The composition of one of the ethanol-crystallized extracts (EC2) was entirely rebaudioside A, whereas the enzymatically modified (EM) extract contained the lowest concentration of this compound (2.7 mg/100 mg). The membrane-purified (MP) extract had the highest content of minor natural steviol glycosides (23.7 mg/100 mg total extract) versus an average of 2.4 mg/100 mg total extract for the EC samples. Thirteen trained panelists evaluated sweetness, bitterness, licorice, and metallic attributes of all four extracts. The highest licorice intensity (p ≤ 0.05) was found for MP. Both samples EC1 and EC2, despite their different chemical compositions, showed no significant differences in sensory perception.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
Sorbitol, European Pharmacopoeia (EP) Reference Standard
Caffeine for system suitability, European Pharmacopoeia (EP) Reference Standard
Supelco
Sucrose, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Sucralose, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Sorbitol, United States Pharmacopeia (USP) Reference Standard
USP
Sucralose, United States Pharmacopeia (USP) Reference Standard
USP
Sucrose, United States Pharmacopeia (USP) Reference Standard
Supelco
Sucralose, analytical standard
Sucralose, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
D-Sorbitol, BioUltra, ≥99.0% (HPLC)
Supelco
Sorbitol, Pharmaceutical Secondary Standard; Certified Reference Material
Sucrose, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
D-Sorbitol, 99% (GC)
Sigma-Aldrich
D-Sorbitol, FCC, FG
Sigma-Aldrich
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Millipore
Sucrose, suitable for microbiology, ACS reagent, ≥99.0%
Sigma-Aldrich
Sucralose, ≥98.0% (HPLC)
Sigma-Aldrich
D-Sorbitol, liquid, tested according to Ph. Eur.
Sigma-Aldrich
Sorbitol F solution, 70 wt. % in H2O, Contains mainly D-sorbitol with lesser amounts of other hydrogenated oligosaccharides
Sigma-Aldrich
D-Sorbitol, ≥98% (GC), BioReagent, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
D-Sorbitol, ≥98% (GC), for molecular biology
Sigma-Aldrich
D-Sorbitol, ≥98% (GC)
Sigma-Aldrich
D-Sorbitol, ≥98% (GC), BioXtra
Sigma-Aldrich
Sucrose, ≥99% (GC), Grade I, suitable for plant cell culture
Sigma-Aldrich
Sucrose, ≥99.5% (GC), BioXtra
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Supelco
Sucrose, analytical standard, for enzymatic assay kit SCA20
Sigma-Aldrich
Sucrose, ACS reagent