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T7513

Thrombin from bovine plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

Synonym(s):

Factor IIa

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50 units
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$132.00
100 units
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$206.00
250 units
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$389.00
500 units
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$602.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
EC Number:
Specific activity:
≥2,000 NIH units/mg protein (E1%/280 = 19.5)

$132.00


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Diagnostics Manufacturers should use alternative grade product: SRE0003

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form

lyophilized powder

Quality Segment

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

UniProt accession no.

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... F2(280685)

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

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1 of 1

This Item
T6634T4648SRE0003
description

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

description

lyophilized powder, 600-2,000 NIH units/mg protein (biuret)

description

lyophilized powder, 40-500 NIH units/mg protein (biuret)

description

Suitable for manufacturing of diagnostic kits and reagents

Gene Information

cow ... F2(280685)

Gene Information

cow ... F2(280685)

Gene Information

cow ... F2(280685)

Gene Information

-

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

specific activity

600-2,000 NIH units/mg protein (biuret)

specific activity

40-500 NIH units/mg protein (biuret)

specific activity

80-500 NIH units/mg protein

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

UniProt accession no.

P00735

UniProt accession no.

P00735

UniProt accession no.

P00735

UniProt accession no.

-

mol wt

light chain ~5 kDa, heavy chain ~33 kDa

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

mol wt

-


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Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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