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Merck

PROTAA-RO

Roche

Protein A Agarose

>98% (HPLC and SDS-PAGE), suspension

Synonim(y):

agarose, protein a

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Wybierz wielkość

2 ML

1910,00 zł

5 ML

3940,00 zł

1910,00 zł

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Informacje o tej pozycji

UNSPSC Code:
41116133
NACRES:
NA.54
Form:
suspension
Assay:
>98% (HPLC and SDS-PAGE)

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assay

>98% (HPLC and SDS-PAGE)

form

suspension

packaging

pkg of 15 mL (05015979001 [settled resin volume]), pkg of 2 mL (11719408001 [settled resin volume]), pkg of 5 mL (11134515001 [settled resin volume])

manufacturer/tradename

Roche

capacity

>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)

storage temp.

2-8°C

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Ta pozycja
16-125PROTGA-ROP1925
Protein A Agarose >98% (HPLC and SDS-PAGE), suspension

Roche

PROTAA-RO

Protein A Agarose

Protein A Agarose Protein A Agarose Beads for the purification of human, mouse & rabbit immunoglobins.

Millipore

16-125

Protein A Agarose

Protein G Agarose >98% (HPLC and SDS-PAGE), suspension

Roche

PROTGA-RO

Protein G Agarose

Protein A–Agarose macrobeads aqueous suspension

Millipore

P1925

Protein A–Agarose macrobeads

assay

>98% (HPLC and SDS-PAGE)

assay

-

assay

>98% (HPLC and SDS-PAGE)

assay

-

form

suspension

form

liquid

form

suspension

form

aqueous suspension

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

packaging

pkg of 15 mL (05015979001 [settled resin volume]), pkg of 5 mL (11134515001 [settled resin volume]), pkg of 2 mL (11719408001 [settled resin volume])

packaging

-

packaging

pkg of 15 mL (05015952001 [settled resin volume]), pkg of 2 mL (11719416001 [settled resin volume]), pkg of 5 mL (11243233001 [settled resin volume])

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Upstate®

manufacturer/tradename

Roche

manufacturer/tradename

-

capacity

>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)

capacity

-

capacity

-

capacity

~6 mg/mL binding capacity (human IgG)

General description

The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times, depending on the elution condition.

Protein A leakage: <18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gel
Protein A, immobilized.

Application

Purification of mouse monoclonal antibodies from ascites fluid or cell culture supernatants. Protein A or Protein G Agarose affinity matrix can be used to purify antibodies from crude cell extracts[1] and for the immunoprecipitation or co-immunoprecipitation of proteins.[2][3][4] It has also been used for photoactivatable-ribonucleoside-enhanced cross-linking immunoprecipitation (PAR-CLIP) analysis.[5]

Biochem/physiol Actions

Protein A is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein A binds to varying degrees IgM, IgA, IgD, and most subclasses of IgG.

Analysis Note

Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.

Other Notes

For life science research only. Not for use in diagnostic procedures.
Preswollen gel, ready-to-use, 2 ml or 5 ml gel (settled gel volume) in buffer that contains 20% ethanol as a preservative, nonsterile.
Protein A content: 3 mg/ml pre-swollen gel
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pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3

Klasa składowania

3 - Flammable liquids

wgk

WGK 1

flash_point_f

102.2 °F

flash_point_c

39 °C

hcodes

H226,H319

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Certyfikaty analizy (CoA)

Lot/Batch Number
10160423
10160422
10090397
10090238
10048051

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